• USA Home
  • 06105 - Bile esculin azide agar

EMAIL THIS PAGE TO A FRIEND
06105 Millipore

Bile esculin azide agar

for microbiology, NutriSelect Plus

Synonym: Enterococcus Selective Agar

  •  NACRES NA.85

Purchase

Properties

Related Categories A - M, Biochemical Identification Media by Test, Biochemical Identification Media, A to Z, Clinical Media, Enterococcus,
Quality Level   100
grade   for microbiology
form   powder
shelf life   limited shelf life, expiry date on the label
composition   agar, 15.0 g/L
  beef extract, 5.0 g/L
  casein enzymic hydrolysate, 17.0 g/L
  esculin, 1.0 g/L
  ferric ammonium citrate, 0.5 g/L
  oxgall, 10.0 g/L
  proteose peptone, 3.0 g/L
  sodium azide, 0.15 g/L
  sodium chloride, 5.0 g/L
mfr. no.   NutriSelect Plus
application(s)   microbiology: suitable (microbial identification)
  water monitoring: suitable
Featured Industry   Clinical Testing
Environmental
Food and Beverages
suitability   selective and differential for Enterococcus spp.
  selective and differential for Streptococcus spp.

Description

General description

Bile esculin agar (BEA) is a differential and selective medium and is mainly used to discern group D Streptococci and Enterococci based on the organism’s potential to hydrolyze esculin. BEA comprises of oxygall (bile salts, first selective ingredients) and azide (second selective ingredients), former inhibits the growth of gram positive organisms and latter inhibits the growth of gram negative organisms. BEA also comprises of nutrients ferric citrate and esculin, where esculin is a differential ingredient and is also a fluorescent compound, upon hydrolyzation the fluorescence is lost. BEA is used for bile esculin test which is based on the hydrolysis of esculin into esculetin (6, 7-dihydroxy-coumarin) and glucose by a micro-organism that produce an enzyme esculinase.

Application

Bile Esculin Azide Agar is a selective medium used for isolation and presumptive identification of faecal Streptococci.

Preparation Note

Suspend 56.65g in 1 l distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 121°C for 15 minutes.

Safety & Documentation

Safety Information

Hazard statements 
Precautionary statements 
Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

Please Enter a Lot Number
Protocols & Articles

Articles

Culture Media and Tests for Microbial Water Testing

Routine testing of water for human consumption is essential to protect the health of consumers. We provide a variety of dehydrated and ready-to-use culture media and tests matching your testing needs.
Keywords: Culture media, Electron capture detector, Indicators

Microbiology Introduction

An initial aim of all microbiologists is the reproducible growth of their microbial cultures, no matter whether the microorganisms are of natural origin or have been genetically engineered by man. Re...
Keywords: Absorption, Aerobic, Anaerobic, Antibiotics, Antimicrobials, Buffers, Culture media, Diffusion, Environmental, Filtration, Genetic, Growth factors, Metabolism, Phase transitions, Precipitation, Reductions, Respiration, Sample preparations, Vitamins

Microbiology Theory: Media Preparation

Microorganisms need nutrients, a source of energy and certain environmental conditions in order to grow and reproduce. In the natural environment, microbes have adapted to the habitats most suitable ...
Keywords: Culture media, Digestions, Environmental, Filtration, Growth factors, Indicators, Microbiology, Phase transitions, Precipitation, Vitamins

Streptococci- Overview of Detection, Identification, Differentiation and Cultivation Techniques

Streptococci are non-motile, microaerophilic, Grampositive spherical bacteria (cocci). They often occur as chains or pairs and are facultative or strict anaerobes. Streptococci give a negative catala...
By Jvo Siegrist, Product Manager Microbiology, ivo.siegrist@sial.com
AnalytiX Volume 7 Article 3
Keywords: Cell division, Diagnostic, Phosphorylations

Peer-Reviewed Papers
15

References

Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?