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09691 Sigma-Aldrich

Ammonium acetate solution

BioUltra, for molecular biology, ~5 M in H2O

  • CAS Number 631-61-8

  • Linear Formula CH3COONH4

  • Molecular Weight 77.08

  •  Beilstein/REAXYS Number 4186741

  •  MDL number MFCD00013066

  •  eCl@ss 39021908

  •  PubChem Substance ID 329748801

  •  NACRES NA.25

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Properties

Related Categories Alphabetical, BioUltra, BioUltra Buffers, Biochemicals and Reagents, Biological Buffers,
Quality Level   200
grade   for molecular biology
product line   BioUltra
form   liquid
concentration   ~5 M in H2O
impurities   DNases, none detected
  RNases, none detected
  insoluble matter, passes filter test
  phosphatases, none detected
  proteases, none detected
ign. residue   ≤0.01% (as SO4)
pH   6.2-7.5 (25 °C, 1 M in H2O)
density   1.07 g/mL at 20 °C
anion traces   chloride (Cl-): ≤5 mg/kg
  nitrate (NO3-): ≤10 mg/kg
  sulfate (SO42-): ≤10 mg/kg
cation traces   Al: ≤1 mg/kg
  As: ≤0.1 mg/kg
  Ba: ≤1 mg/kg
  Bi: ≤1 mg/kg
  Ca: ≤5 mg/kg
  Cd: ≤1 mg/kg
  Co: ≤1 mg/kg
  Cr: ≤1 mg/kg
  Cu: ≤1 mg/kg
  Fe: ≤1 mg/kg
  K: ≤20 mg/kg
  Li: ≤1 mg/kg
  Mg: ≤1 mg/kg
  Mn: ≤1 mg/kg
  Mo: ≤1 mg/kg
  Na: ≤20 mg/kg
  Ni: ≤1 mg/kg
  Pb: ≤1 mg/kg
  Sr: ≤1 mg/kg
  Zn: ≤1 mg/kg
λ   1 M in H2O
UV absorption   λ: 260 nm Amax: 0.015
  λ: 280 nm Amax: 0.01
SMILES string   CC([O-])=O.[H][N+]([H])([H])[H]
InChI   1S/C2H4O2.H3N/c1-2(3)4;/h1H3,(H,3,4);1H3
InChI key   USFZMSVCRYTOJT-UHFFFAOYSA-N

Description

Application

Ammonium acetate solution can be used to study molecular biology, biological buffers, reagents and DNA and RNA purification. Ammonium acetate solution has been used to study pharmacokinetic analysis of α and β epimers of glycyrrhetinic acid in rat plasma. Ammonium acetate solution has also been used in a study to develop a method for the simultaneous determination of aristolochic acids A and B in some Chinese herbals and traditional Chinese patent medicines.

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
1

Documents

Certificate of Analysis (COA)

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Single- & multi-channel pipettes from BRAND
Protocols & Articles

Protocols

Protocol for Anti Ago-RNA Immunoprecipitation from mammalian cells using the RIP kit

1.    Grow cells to ~80% confluency.  You will need ~2 million cells/RIP, or ~4 million cells for IgG (neg control) & Ago2 RIP
Keywords: Cell disruption, Immunoprecipitation, Individual protein Immunoprecipitation, RNA immunoprecipitation

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Peer-Reviewed Papers
15

References

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