• USA Home
  • 09743 - 3,3′,5,5′-Tetramethylbenzidine

EMAIL THIS PAGE TO A FRIEND
09743 Supelco

3,3′,5,5′-Tetramethylbenzidine

standard for GC

Synonym: BM blue, Sure Blue TMB, TMB Blotting Plus, TMB substrate, TMB

  • CAS Number 54827-17-7

  • Linear Formula [-C6H2(CH3)2-4-NH2]2

  • Molecular Weight 240.34

  •  Beilstein/REAXYS Number 2808541

  •  EC Number 259-364-6

  •  MDL number MFCD00007748

  •  PubChem Substance ID 329748813

  •  NACRES NA.24

Purchase

Properties

Related Categories 2002/61/EC, Additional Standards, Alpha Sort, Analytical Standards, Analytical/Chromatography,
Quality Level   100
grade   analytical standard
  standard for GC
assay   ≥99.0% (GC)
shelf life   limited shelf life, expiry date on the label
application(s)   HPLC: suitable
  gas chromatography (GC): suitable
mp   166-171 °C
  168-171 °C (lit.)
Featured Industry   Environmental
SMILES string   Cc1cc(cc(C)c1N)-c2cc(C)c(N)c(C)c2
InChI   1S/C16H20N2/c1-9-5-13(6-10(2)15(9)17)14-7-11(3)16(18)12(4)8-14/h5-8H,17-18H2,1-4H3
InChI key   UAIUNKRWKOVEES-UHFFFAOYSA-N

Description

Application

3,3′,5,5′-Tetramethylbenzidine (TMB) is a noncarcinogenic substitute (Ames test negative) for benzidine suitable as peroxidase substrate for use in ELISA procedures. The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 620-650 nm. The TMB reaction may be stopped with 2 M H2SO4 (resulting in a yellow color), and read at 450 nm. A sensitive and specific reagent for the detection of blood, assay of hemoglobin, assay of peroxidases.

Other Notes

this product will be discontinued, please find the alternative product here :
860336

Standard for determining aromatic amines released from azo-dyes in commodities

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
RTECS 
DV2300000
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

Please Enter a Lot Number
Protocols & Articles
Peer-Reviewed Papers
15

References

Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?