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09830 Sigma-Aldrich

Ammonium bicarbonate

BioUltra, ≥99.5% (T)

Synonym: Ammonium hydrogen carbonate

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Properties

Related Categories Alphabetical, BioUltra Buffers, Biochemicals and Reagents, Biological Buffers
vapor density   2.7 (vs air)
InChI Key   ATRRKUHOCOJYRX-UHFFFAOYSA-N
product line   BioUltra
assay   ≥99.5% (T)
impurities   insoluble matter, passes filter test
pH   7.0-8.5 (25 °C, 1 M in H2O)
solubility   H2O: 1 M at 20 °C, clear, colorless
anion traces   chloride (Cl-): ≤50 mg/kg
  sulfate (SO42-): ≤50 mg/kg
cation traces   Al: ≤5 mg/kg
  As: ≤0.1 mg/kg
  Ba: ≤5 mg/kg
  Bi: ≤5 mg/kg
  Ca: ≤10 mg/kg
  Cd: ≤5 mg/kg
  Co: ≤5 mg/kg
  Cr: ≤5 mg/kg
  Cu: ≤5 mg/kg
  Fe: ≤5 mg/kg
  K: ≤50 mg/kg
  Li: ≤5 mg/kg
  Mg: ≤500 mg/kg
  Mn: ≤5 mg/kg
  Mo: ≤5 mg/kg
  Na: ≤50 mg/kg
  Ni: ≤5 mg/kg
  Pb: ≤5 mg/kg
  Sr: ≤5 mg/kg
  Zn: ≤5 mg/kg
λ   1 M in H2O
UV absorption   λ: 260 nm Amax: ≤0.030
  λ: 280 nm Amax: ≤0.020

Description

Other Notes

Sample loading solution for the gas-phase sequenator; Removable eluant in ion-exchange chromatography; Buffer component for the digestion of tRNA.

Application

Ammonium bicarbonate can be used to study biological buffers. Ammonium bicarbonate has been used in a study that demonstrated that ammonium bicarbonate salts, which can be regenerated using low-temperature waste heat, can also produce sufficient voltage for hydrogen gas generation in a microbial reverse-electrodialysis electrolysis cells. Ammonium bicarbonate has also been used in a study that developed a fast and sensitive method for the simultaneous determination of Sudan dyes in food samples using partial filling micellar electrokinectic chromatography-mass spectrometry.

Safety & Documentation

Safety Information

Symbol 
GHS07  GHS07
Signal word 
Warning
Hazard statements 
Precautionary statements 
Personal Protective Equipment 
WGK Germany 
1
RTECS 
BO8600000
Protocols & Articles

Protocols

In-gel digestion for mass spectrometric characterization of proteins and proteomes

In-gel digestion of proteins isolated by gel electrophoresis is a cornerstone of mass spectrometry (MS)-driven proteomics. The 11-year-old recipe by Shevchenko et al. has been optimized to increase t...
Keywords: 2D Electrophoresis, Alkylations, Biochemistry, Cell biology, Cell culture, Cell disruption, Chromatography, Condensations, Dehydration reaction, Detergents, Diffusion, Digestions, Electrophoresis, Gel electrophoresis, Gene expression, Genetics, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Phase transitions, Polymerase chain reaction, Proteomics, Reductions, Separation, Sequencing, Sonication

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BioUltra Biological Buffers

A buffer, as defined by Van Slyke [1], is "a substance which by its presence in solution increases the amount of acid or alkali that must be added to cause unit change in pH". Buffers are thus very i...
Keywords: Absorption, Adsorption, Biochemistry, Biological Buffers, Biological processes, Buffers, PAGE

Peer-Reviewed Papers
15

References

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