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114375 Sigma-Aldrich

Bromocresol Purple

BioReagent, suitable for indicator, Dye content 90 %

Synonym: 5,5′-Dibromo-o-cresolsulfonphthalein, Bromcresol purple sultone form

  • CAS Number 115-40-2

  • Empirical Formula (Hill Notation) C21H16Br2O5S

  • Molecular Weight 540.22

  •  Beilstein Registry Number 359618

  •  EC Number 204-087-8

  •  MDL number MFCD00011681

  •  PubChem Substance ID 24847168

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Properties

Related Categories Analytical Reagents, Analytical/Chromatography, B, Cell Biology, Hematology and Histology,
InChI Key   ABIUHPWEYMSGSR-UHFFFAOYSA-N
product line   BioReagent
form   powder
composition   Dye content, 90%
pH range   5.2 - 6.8, yellow to purple
visual transition interval   5.2 - 6.8, yellow to blue
mp   240 °C (dec.) (lit.)
solubility   ethanol: 1 mg/mL
absorption   extinction/418-424 nm, methanol ≥22000
  λmax 419 nm
suitability   suitable for indicator

Description

Application

Spectrophotometric determination of serum-albumin

Bromocresol Purple has been used for staining of Saccharomyces cerevisiae.

Packaging

5, 25 g in glass bottle

Biochem/physiol Actions

Bromocresol purple is a pH indicator which changes color from yellow (at low pH 5.2) to violet (above pH 6.8). It is mainly used as a fluorescent stain in yeast cells. It helps in dead cell count for cells with plasma membrane damage. Cells with proper cell membrane are not stained, while damaged cells are seen as blue-grey ghosts.

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin


Milli-Q® Water Purification Solutions
Protocols & Articles
Peer-Reviewed Papers
15

References

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