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114375 Sigma-Aldrich

Bromocresol Purple

BioReagent, suitable for indicator, Dye content 90 %

Synonym: 5,5′-Dibromo-o-cresolsulfonphthalein, Bromcresol purple sultone form

  • CAS Number 115-40-2

  • Empirical Formula (Hill Notation) C21H16Br2O5S

  • Molecular Weight 540.22

  •  Beilstein/REAXYS Number 359618

  •  EC Number 204-087-8

  •  MDL number MFCD00011681

  •  PubChem Substance ID 24847168

  •  NACRES NA.47

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Properties

Related Categories Analytical Reagents, Analytical/Chromatography, B, Cell Biology, Hematology and Histology,
Quality Level   200
product line   BioReagent
assay   ≥89.0% (HPLC)
form   powder
composition   Dye content, 90%
application(s)   hematology: suitable
  histology: suitable
  titration: suitable
pH range   5.2 - 6.8, yellow to purple
visual transition interval   5.2 - 6.8, yellow to blue
mp   240 °C (dec.) (lit.)
solubility   ethanol: 1 mg/mL
λmax   419 nm
ε (extinction coefficient)   ≥22000 at 418-424 nm in methanol
suitability   suitable for indicator
Featured Industry   Diagnostic Assay Manufacturing
storage temp.   room temp
SMILES string   Cc1cc(cc(Br)c1O)C2(OS(=O)(=O)c3ccccc23)c4cc(C)c(O)c(Br)c4
InChI   1S/C21H16Br2O5S/c1-11-7-13(9-16(22)19(11)24)21(14-8-12(2)20(25)17(23)10-14)15-5-3-4-6-18(15)29(26,27)28-21/h3-10,24-25H,1-2H3
InChI key   ABIUHPWEYMSGSR-UHFFFAOYSA-N

Description

Application

Bromocresol Purple has been used for staining of Saccharomyces cerevisiae.

Spectrophotometric determination of serum-albumin

Packaging

5, 25 g in glass bottle

Biochem/physiol Actions

Bromocresol purple is a pH indicator which changes color from yellow (at low pH 5.2) to violet (above pH 6.8). It is mainly used as a fluorescent stain in yeast cells. It helps in dead cell count for cells with plasma membrane damage. Cells with proper cell membrane are not stained, while damaged cells are seen as blue-grey ghosts.

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles
Peer-Reviewed Papers
15

References

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