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131164 Sigma-Aldrich

Indigo carmine

certified by the Biological Stain Commission, Dye content 85 %

Synonym: Acid Blue 74, Indigo-5,5′-disulfonic acid disodium salt, Indigocarmine

  • CAS Number 860-22-0

  • Empirical Formula (Hill Notation) C16H8N2Na2O8S2

  • Molecular Weight 466.35

  •  Colour Index Number 73015

  •  Beilstein/REAXYS Number 4103904

  •  EC Number 212-728-8

  •  MDL number MFCD00005723

  •  PubChem Substance ID 24847997

  •  NACRES NA.47

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Properties

Related Categories Cell Biology, Certified Stains & Dyes, G-H-I, Hematology and Histology, Stains & Dyes, A to Z,
Quality Level   200
grade   certified by the Biological Stain Commission
assay   >85.0% (HPLC)
form   solid
composition   Dye content, 85%
application(s)   hematology: suitable
  histology: suitable
pH   3.87 (30 °C, 7.14 g/L)
mp   ≥340 °C
density   0.71 g/cm3 at 29 °C
λmax   608 nm
εmax   20,000 at 250-256 nm in water at 0.01 g/L
  29500 at 284-290 nm in water at 0.01 g/L
Featured Industry   Diagnostic Assay Manufacturing
storage temp.   room temp
SMILES string   [Na+].[Na+].[O-]S(=O)(=O)c1ccc2NC(\C(=O)c2c1)=C3\Nc4ccc(cc4C3=O)S([O-])(=O)=O
InChI   1S/C16H10N2O8S2.2Na/c19-15-9-5-7(27(21,22)23)1-3-11(9)17-13(15)14-16(20)10-6-8(28(24,25)26)2-4-12(10)18-14;;/h1-6,17-18H,(H,21,22,23)(H,24,25,26);;/q;2*+1/p-2/b14-13+;;
InChI key   KHLVKKOJDHCJMG-QDBORUFSSA-L

Description

Application

Indigo carmine has been used to investigate its effects on ozone production by neutrophils.

Packaging

25, 100 g in glass bottle

Biochem/physiol Actions

Indigo Carmine (IC), commonly known as acid blue 74 or food blue 1 or FD and C blue 2 is a blue synthetic dye. It is used as a redox indicator in analytical chemistry and as a microscopic stain in biology. IC is also employed as a photometric detector. IC is a highly toxic indigoid dye and is associated with various health hazards such as irritation to the gastrointestinal tract leading to nausea, vomiting, and diarrhea and irritation in the respiratory tract leading to coughing and shortness of breath. IC exposure might also cause skin and eye irritations to humans. IC dye spray is used for the diagnosis of dysplasia in ulcerative colitis and is considered to be a simple, feasible, and safe method.

Suitability

Used as a collagen stain and in procedures using red nuclear stains.

Safety & Documentation

Safety Information

Symbol 
GHS07  GHS07
Signal word 
Warning
Hazard statements 
Precautionary statements 
Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 1
RTECS 
DU3000000

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

Articles

HPLC Analysis of Synthetic Food Dyes on Ascentis® Express C18

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Synthetic Food Dyes on Ascentis® Express C18
Keywords: Chromatography, High performance liquid chromatography

Peer-Reviewed Papers
15

References

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