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15604 Sigma-Aldrich

Urea

puriss., meets analytical specification of Ph. Eur., BP, USP, 99.0-100.5%, 99.0-101.0% (calc. on dry substance)

Synonym: Carbamide, Carbonyldiamide

  • CAS Number 57-13-6

  • Linear Formula NH2CONH2

  • Molecular Weight 60.06

  •  Beilstein/REAXYS Number 635724

  •  EC Number 200-315-5

  •  MDL number MFCD00008022

  •  PubChem Substance ID 329750941

  •  NACRES NA.21

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Properties

Quality Level   200
grade   puriss.
assay   99.0-100.5%
  99.0-101.0% (calc. on dry substance)
form   solid
quality   meets analytical specification of Ph. Eur., BP, USP
impurities   alkalic reac. substances, complies
  ≤0.001% heavy metals (as Pb)
  ≤0.04% insoluble in ethanol
  ≤0.05% ammonium (NH4)
  ≤0.1% biuret(carbamylurea)
ign. residue   ≤0.1% (as SO4)
loss   ≤1.0% loss on drying, 105 °C, 1 h
mp   132-135 °C (lit.)
  132-135 °C
solubility   H2O: soluble 480 g/L at 20 °C
density   1.335 g/mL at 25 °C (lit.)
anion traces   chloride (Cl-): ≤10 mg/kg
  sulfate (SO42-): ≤50 mg/kg
cation traces   Fe: ≤0.5 mg/kg
suitability   in accordance for appearance of solution
  in accordance for identity
SMILES string   NC(N)=O
InChI   1S/CH4N2O/c2-1(3)4/h(H4,2,3,4)
InChI key   XSQUKJJJFZCRTK-UHFFFAOYSA-N

Description

General description

Urea is a colorless, odorless solid. It has prism-like crystals. It forms insoluble nitrate salts with nitric acid and oxalate salts with oxalic acid. On reaction with alkanes or substituted alkanes, it affords crystal-lattice inclusion compounds.
Urea can be prepared from ammonia and carbon-dioxide, via Haber-Bosch process. It can also be used to prepare urea-formaldehyde resins, via condensation reaction.

Application

Urea has been used in the following studies:
• Synthesis of SnO2 nanoparticles.
• Synthesis of urethanes, ureides and semicarbazides.
• Denaturation of protein isolated from Saccharomyces cerevisiae Y15696 strain
• Protein electrophoretic studies.

Used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form.

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 1
RTECS 
YR6250000
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Protocols & Articles
Peer-Reviewed Papers
15

References

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