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17153 Millipore

LS Differential Agar

for microbiology

Synonym: Lactobacillus Streptococcus Differential Agar

  •  NACRES NA.85

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Properties

Related Categories A - M, All Dairy Media (alphabetical), All Solid Media/Agars, Lactobacillus, Media by Application,
Quality Level   200
grade   for microbiology
form   powder
shelf life   limited shelf life, expiry date on the label
composition   agar, 15 g/L
  beef extract, 5 g/L
  casein enzymic hydrolysate, 10 g/L
  L-cysteine hydrochloride, 0.3 g/L
  dextrose, 20 g/L
  papaic digest of soybean meal, 5 g/L
  sodium chloride, 5 g/L
  yeast extract, 5 g/L
application(s)   bioburden testing: suitable
  microbiological culture: suitable
final pH   6.1±0.2 (25 °C)
Featured Industry   Environmental
Food and Beverages
suitability   nonselective and differential for Lactobacillus spp.
  nonselective and differential for Streptococcus spp.

Description

Application

For the maximum growth and differentiation of Lactobacilli and Streptococci on the basis of colonial morphology, T.T.C. reduction and casein reaction.

Preparation Note

Supplements:
100ml/1.11l 10% (w/v) aqueous solution of antibiotic free skim milk powder (Cat. No. 70166)
10 ml/1.11l TTC Solution (Cat. No. 17779)

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Microbiological Media and Detection

Molecular biology-based methods, like PCR, can be used for lactobacilli detection. However, they are often quite expensive. Sigma-Aldrich provides a revolutionary molecular biology method that is rap...
Chloe McClanahan
BioFiles Edition 6.2
Keywords: Amplification, Anaerobic, Antimicrobials, Magnetic resonance spectroscopy, Molecular biology, Nucleic acid hybridization, Polymerase chain reaction, Reductions

Microbiology Introduction

An initial aim of all microbiologists is the reproducible growth of their microbial cultures, no matter whether the microorganisms are of natural origin or have been genetically engineered by man. Re...
Keywords: Absorption, Aerobic, Anaerobic, Antibiotics, Antimicrobials, Buffers, Culture media, Diffusion, Environmental, Filtration, Genetic, Growth factors, Metabolism, Phase transitions, Precipitation, Reductions, Respiration, Sample preparations, Vitamins

Microbiology Theory: Media Preparation

Microorganisms need nutrients, a source of energy and certain environmental conditions in order to grow and reproduce. In the natural environment, microbes have adapted to the habitats most suitable ...
Keywords: Culture media, Digestions, Environmental, Filtration, Growth factors, Indicators, Microbiology, Phase transitions, Precipitation, Vitamins

Probiotics and Human Health

At the beginning of the 20th century, probiotics were thought to have a beneficial effect to the host by improving the intestinal microbial balance. Through his studies and observations, the Russian ...
Chloe McClanahan
BioFiles Edition 6.2
Keywords: AGE, Absorption, Adhesion, Amplification, Anaerobic, Antimicrobials, Cancer, Carcinogens, Cell proliferation, Chromatography, Coagulation, Detoxification, Diagnostic, Diseases, Enzyme activity, Fermentation, Gas chromatography, Gastrointestinal, High performance liquid chromatography, Inflammation, Magnetic resonance spectroscopy, Metabolism, Metabolites, Molecular biology, Nucleic acid hybridization, PAGE, Pharmaceutical, Phosphorylations, Polymerase chain reaction, Prebiotics, Probiotics, Reductions, Respiratory, Sequencing, Thin layer chromatography, Titrations

Streptococci- Overview of Detection, Identification, Differentiation and Cultivation Techniques

Streptococci are non-motile, microaerophilic, Grampositive spherical bacteria (cocci). They often occur as chains or pairs and are facultative or strict anaerobes. Streptococci give a negative catala...
By Jvo Siegrist, Product Manager Microbiology, ivo.siegrist@sial.com
AnalytiX Volume 7 Article 3
Keywords: Cell division, Diagnostic, Phosphorylations

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