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  • 17170 - AEA Sporulation Broth (Base), modified

17170 Millipore

AEA Sporulation Broth (Base), modified

suitable for microbiology, NutriSelect Plus

  •  NACRES NA.85



Related Categories A - M, All Food Control Media (alphabetical), All Liquid Media/Broths, Clostridia, Media by Application,
Quality Level   100
form   powder
shelf life   limited shelf life, expiry date on the label
composition   ammonium acetate, 1.5 g/L
  biopeptone, 10 g/L
  disodium phosphate, 4.36 g/L
  magnesium sulfate.7H2O, 0.2 g/L
  monopotassium phosphate, 0.25 g/L
  yeast extract, 10 g/L
mfr. no.   NutriSelect Plus
application(s)   microbiological culture: suitable
  microbiology: suitable (microbial identification)
  microbiology: suitable
final pH   7.8±0.2 (25 °C)
Featured Industry   Food and Beverages
storage temp.   room temp
suitability   nonselective for Clostridium spp.



For early sporulation of Clostridium perfringens from foods.

Preparation Note

Suspend 26.31 g in 1 litre distilled water. Dispense the medium in 15 ml amounts in screw capped tubes and sterilize by autoclaving at 121°C for 15 minutes. Cool to 45-50°C and add 0.6 ml of filter sterilized 10% raffinose and 0.2 ml each of sterile 0.66 M sodium carbonate and 0.32% cobalt chloride dropwise to each 15 ml base medium in the tubes. Just before use, steam the medium for 10 minutes and after cooling, add 0.2 ml of filter sterilized (freshly prepared) 1.5% sodium ascorbate to each tube of the medium.

Legal Information

NutriSelect is a trademark of Merck KGaA, Darmstadt, Germany

Safety & Documentation

Safety Information

Personal Protective Equipment 
NONH for all modes of transport
WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable


Certificate of Analysis (COA)

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Protocols & Articles


Clostridia Diagnostic

Clostridia are relatively large, gram-positive, rod-shaped bacteria that can undergo only anaerobic metabolism. Most Clostridia cannot grow under aerobic conditions and even can be killed by exposure...
Jvo Siegrist
AnalytiX Volume 7 Article 2
Keywords: Aerobic, Anaerobic, Clinical, Degradations, Environmental, Fermentation, Germination, Gram stain, Indicators, Metabolism, Microbiology, Pharmaceutical, Vitamins

Clostridium perfringens, their Properties and their Detection

Clostridia are relatively large, Gram-positive, rod-shaped bacteria that can undergo only anaerobic metabolism. Most Clostridia cannot grow under aerobic conditions and can even be killed by exposure...
AnalytiX Volume 10 Article 4
Keywords: Aerobic, Anaerobic, Chlorinations, Degradations, Environmental, Fermentation, Germination, Gram stain, Indicators, Metabolism, Microbiology

Microbiology Introduction

An initial aim of all microbiologists is the reproducible growth of their microbial cultures, no matter whether the microorganisms are of natural origin or have been genetically engineered by man. Re...
Keywords: Absorption, Aerobic, Anaerobic, Antibiotics, Antimicrobials, Buffers, Culture media, Diffusion, Environmental, Filtration, Genetic, Growth factors, Metabolism, Phase transitions, Precipitation, Reductions, Respiration, Sample preparations, Vitamins

Microbiology Theory: Media Preparation

Microorganisms need nutrients, a source of energy and certain environmental conditions in order to grow and reproduce. In the natural environment, microbes have adapted to the habitats most suitable ...
Keywords: Culture media, Digestions, Environmental, Filtration, Growth factors, Indicators, Microbiology, Phase transitions, Precipitation, Vitamins

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