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  • 17173 - Rappaport Vassiliadis Broth, modified

17173 Millipore

Rappaport Vassiliadis Broth, modified

for microbiology, NutriSelect Plus

Synonym: Magnesium chloride Malachite Green Broth, Modified Rappaport Vassiliadis Broth, RV Broth, Salmonella Enrichment Broth acc. to Rappaport and Vassiliadis

  •  NACRES NA.85



Related Categories All Dairy Media (alphabetical), All Food Control Media (alphabetical), All Liquid Media/Broths, Media by Application, Media by Microorganism,
Quality Level   100
grade   for microbiology
form   powder
shelf life   limited shelf life, expiry date on the label
mfr. no.   NutriSelect Plus
application(s)   microbiological culture: suitable
  pathogen testing: suitable
final pH   5.2±0.2 (25 °C)
Featured Industry   Environmental
Food and Beverages
Food and Beverages
suitability   selective for Salmonella spp.



Used as a selective enrichment medium for the isolation of Salmonella species from food and environmental specimens.


Papaic digest of soyabean meal 5 g/1110 mL;
Sodium chloride 8 g/1110 mL;
Monopotassium phosphate 1.6 g/1110 mL;
Magnesium chloride anhydrous 18.7 g/l (is equivalent to 40 g/1110 mL Magnesium chloride hexahydrate);
Malachite Green 0.04 g/1110 ml


Suspend 30.0 g in 1 liter distilled water. Heat gently if necessary to dissolve the medium completely. Dispense as desired into tubes and sterilize by autoclaving at 115 °C for 15 minutes.

Safety & Documentation

Safety Information

Hazard statements 
Precautionary statements 
Personal Protective Equipment 
NONH for all modes of transport
WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable


Certificate of Analysis (COA)

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Protocols & Articles


Differentiation and Identification Media for Salmonella

Salmonella contamination is the second leading cause of food-borne illness worldwide. Controlling outbreaks of Salmonella is an important task for food regulators, restaurants and the food industry i...
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Keywords: Biochemistry, Fermentation, Food & Beverage, Indicators, Microbiology

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An initial aim of all microbiologists is the reproducible growth of their microbial cultures, no matter whether the microorganisms are of natural origin or have been genetically engineered by man. Re...
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Microbiology Theory: Media Preparation

Microorganisms need nutrients, a source of energy and certain environmental conditions in order to grow and reproduce. In the natural environment, microbes have adapted to the habitats most suitable ...
Keywords: Culture media, Digestions, Environmental, Filtration, Growth factors, Indicators, Microbiology, Phase transitions, Precipitation, Vitamins

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