17222 Millipore

WL Nutrient Agar

for microbiology

Synonym: Wallerstein Laboratory Nutrient Agar

  •  NACRES NA.74



Related Categories All Solid Media/Agars, Bacteria (General Media), Candida, Escherichia coli & Coliforms, Lactobacillus,
Quality Level   100
grade   for microbiology
form   powder
shelf life   limited shelf life, expiry date on the label
composition   agar, 20 g/L
  bromocresol green, 0.022 g/L
  calcium chloride, 0.125 g/L
  casein enzymic hydrolysate, 5 g/L
  dextrose, 50 g/L
  ferric chloride, 0.0025 g/L
  magnesium sulfate, 0.125 g/L
  manganese sulfate, 0.0025 g/L
  monopotassium phosphate, 0.55 g/L
  potassium chloride, 0.425 g/L
  yeast extract, 4 g/L
application(s)   bioburden testing: suitable
  microbiological culture: suitable
final pH   5.5±0.2 (25 °C)
Featured Industry   Food and Beverages
suitability   nonselective and differential for Candida spp.
  nonselective and differential for Escherichia coli
  nonselective and differential for Lactobacillus spp.
  nonselective and differential for Pichia spp.
  nonselective and differential for Proteus spp.
  nonselective and differential for Saccharomyces spp.
  nonselective and differential for Zygosaccharomyces spp.
  nonselective and differential for bacteria (General Media)
  nonselective and differential for coliforms
  nonselective and differential for molds (General Media)
  nonselective and differential for yeasts (General Media)


General description

WL (Walerstein Laboratory) media follow the formula described by Green and Gray for the examination of materials related to brewing and for industrial fermentations containing mixed flora of yeasts and bacteria. Yeast extract is a source of vitamins, amino acids and trace elements. Casein enzymic hydrolysate provides nitrogen, amino acids and carbon. Dextrose is the main carbon and energy source. Potassium phosphate is the buffering agent. Several salts in the medium provide essential ions and help to maintain osmotic balance. Bromcresol green acts as pH indicator and agar as solidifying agent. WL Nutrient Agar is used for the examination of materials encountered in brewing and for industrial fermentations containing mixed flora of yeasts and bacteria.


WL Nutrient Agar is suitable:
• for reviving yeast stock cultures and for population dynamics studies post fermentation
• for morphological characterization of yeast isolated from cheese
• for plating fermented wine for yeast species isolation

Safety & Documentation

Safety Information

Personal Protective Equipment 
NONH for all modes of transport
WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable


Certificate of Analysis (COA)

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