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17222 Millipore

WL Nutrient Agar

for microbiology

Synonym: Wallerstein Laboratory Nutrient Agar

  •  NACRES NA.74

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Properties

Related Categories All Solid Media/Agars, Bacteria (General Media), Candida, Escherichia coli & Coliforms, Lactobacillus,
Quality Level   100
grade   for microbiology
form   powder
shelf life   limited shelf life, expiry date on the label
composition   agar, 20 g/L
  bromocresol green, 0.022 g/L
  calcium chloride, 0.125 g/L
  casein enzymic hydrolysate, 5 g/L
  dextrose, 50 g/L
  ferric chloride, 0.0025 g/L
  magnesium sulfate, 0.125 g/L
  manganese sulfate, 0.0025 g/L
  monopotassium phosphate, 0.55 g/L
  potassium chloride, 0.425 g/L
  yeast extract, 4 g/L
application(s)   bioburden testing: suitable
  microbiological culture: suitable
final pH   5.5±0.2 (25 °C)
Featured Industry   Food and Beverages
suitability   nonselective and differential for Candida spp.
  nonselective and differential for Escherichia coli
  nonselective and differential for Lactobacillus spp.
  nonselective and differential for Pichia spp.
  nonselective and differential for Proteus spp.
  nonselective and differential for Saccharomyces spp.
  nonselective and differential for Zygosaccharomyces spp.
  nonselective and differential for bacteria (General Media)
  nonselective and differential for coliforms
  nonselective and differential for molds (General Media)
  nonselective and differential for yeasts (General Media)

Description

General description

WL (Walerstein Laboratory) media follow the formula described by Green and Gray for the examination of materials related to brewing and for industrial fermentations containing mixed flora of yeasts and bacteria. Yeast extract is a source of vitamins, amino acids and trace elements. Casein enzymic hydrolysate provides nitrogen, amino acids and carbon. Dextrose is the main carbon and energy source. Potassium phosphate is the buffering agent. Several salts in the medium provide essential ions and help to maintain osmotic balance. Bromcresol green acts as pH indicator and agar as solidifying agent. WL Nutrient Agar is used for the examination of materials encountered in brewing and for industrial fermentations containing mixed flora of yeasts and bacteria.

Application

WL Nutrient Agar is suitable:
• for reviving yeast stock cultures and for population dynamics studies post fermentation
• for morphological characterization of yeast isolated from cheese
• for plating fermented wine for yeast species isolation

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Culture Media and Tests for Beverage Testing

Spoilage organism testing is a crucial step within the beverage industry to enable the safe release of drinks to the market. Requirements can vary from industry to industry, depending on different sp...
Keywords: Culture media, Fermentation, Magnetic resonance spectroscopy

Culture Method for Quality Control of Beer

Traditional methods are based morphology, staining methods, enzyme reactions (metabolism) and diverse media. One of the first steps is the filtration of the beer followed by an incubation of the filt...
Jvo Siegrist
Microbiology Focus Edition 1.4
Keywords: Fermentation, Filtration, Magnetic resonance spectroscopy, Metabolism, Microbiology

Microbiological Media and Detection

Molecular biology-based methods, like PCR, can be used for lactobacilli detection. However, they are often quite expensive. Sigma-Aldrich provides a revolutionary molecular biology method that is rap...
Chloe McClanahan
BioFiles Edition 6.2
Keywords: Amplification, Anaerobic, Antimicrobials, Magnetic resonance spectroscopy, Molecular biology, Nucleic acid hybridization, Polymerase chain reaction, Reductions

Microbiology Introduction

An initial aim of all microbiologists is the reproducible growth of their microbial cultures, no matter whether the microorganisms are of natural origin or have been genetically engineered by man. Re...
Keywords: Absorption, Aerobic, Anaerobic, Antibiotics, Antimicrobials, Buffers, Culture media, Diffusion, Environmental, Filtration, Genetic, Growth factors, Metabolism, Phase transitions, Precipitation, Reductions, Respiration, Sample preparations, Vitamins

Microbiology Theory: Media Preparation

Microorganisms need nutrients, a source of energy and certain environmental conditions in order to grow and reproduce. In the natural environment, microbes have adapted to the habitats most suitable ...
Keywords: Culture media, Digestions, Environmental, Filtration, Growth factors, Indicators, Microbiology, Phase transitions, Precipitation, Vitamins

Probiotics and Human Health

At the beginning of the 20th century, probiotics were thought to have a beneficial effect to the host by improving the intestinal microbial balance. Through his studies and observations, the Russian ...
Chloe McClanahan
BioFiles Edition 6.2
Keywords: AGE, Absorption, Adhesion, Amplification, Anaerobic, Antimicrobials, Cancer, Carcinogens, Cell proliferation, Chromatography, Coagulation, Detoxification, Diagnostic, Diseases, Enzyme activity, Fermentation, Gas chromatography, Gastrointestinal, High performance liquid chromatography, Inflammation, Magnetic resonance spectroscopy, Metabolism, Metabolites, Molecular biology, Nucleic acid hybridization, PAGE, Pharmaceutical, Phosphorylations, Polymerase chain reaction, Prebiotics, Probiotics, Reductions, Respiratory, Sequencing, Thin layer chromatography, Titrations

Peer-Reviewed Papers
15

References

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