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199664 Sigma-Aldrich

Sudan Black B

certified by the Biological Stain Commission

Synonym: Ceres black BN, Fat Black HB, Solvent Black 3

  • CAS Number 4197-25-5

  • Empirical Formula (Hill Notation) C29H24N6

  • Molecular Weight 456.54

  •  Colour Index Number 26150

  •  Beilstein/REAXYS Number 723248

  •  EC Number 224-087-1

  •  MDL number MFCD00006919

  •  PubChem Substance ID 24851932

  •  NACRES NA.47

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Properties

Related Categories Cell Biology, Certified Stains & Dyes, Hematology and Histology, S, Stains & Dyes, A to Z,
Quality Level   200
grade   certified by the Biological Stain Commission
form   powder
application(s)   hematology: suitable
  histology: suitable
mp   120-124 °C (lit.)
λmax   415 nm (2nd)
  598 nm
ε (extinction coefficient)   ≥20000 at 596-603 nm in ethanol
Featured Industry   Diagnostic Assay Manufacturing
storage temp.   room temp
SMILES string   CC1(C)Nc2cccc3c(ccc(N1)c23)\N=N\c4ccc(\N=N\c5ccccc5)c6ccccc46
InChI   1S/C29H24N6/c1-29(2)30-26-14-8-13-22-25(17-18-27(31-29)28(22)26)35-34-24-16-15-23(20-11-6-7-12-21(20)24)33-32-19-9-4-3-5-10-19/h3-18,30-31H,1-2H3/b33-32+,35-34+
InChI key   YCUVUDODLRLVIC-VPHDGDOJSA-N

Description

Application

Sudan Black B has been used in brain BLAQ (Block Lipids and Aldehyde Quench) technique and is used in lipid detection.

Packaging

25 g in glass bottle

Biochem/physiol Actions

Sudan Black B has been used to stain phospholipids and intracellular lipids. Staining pattern is almost similar to myeloperoxidase reaction, with positive staining of granulocytic cells, eosinophils and weak monocytic staining.It doesn’t stain lymphocytes. Sudan Black B can be used to stain older blood or bone marrow smears, but the stain slightly fades over time. It is considered to be a most sensitive and specific reagent for lipid staining. Sudan Black B stains phospholipids gray and neutral fats blue-black.

Suitability

Certified as a fat staining using the Churukian variant of the Lillie-Ashburn supersaturated isopropanol method. Sudan III is substituted for the oil red O in the procedure. Burdon′s stain for bacterial lipid.

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
RTECS 
SD4431500
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles
Peer-Reviewed Papers
15

References

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