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252859 Sigma-Aldrich

Tris(hydroxymethyl)aminomethane

ACS reagent, ≥99.8%

Synonym: 2-Amino-2-(hydroxymethyl)-1,3-propanediol, THAM, Tris base, Trometamol

  • CAS Number 77-86-1

  • Linear Formula NH2C(CH2OH)3

  • Molecular Weight 121.14

  •  Beilstein/REAXYS Number 741883

  •  EC Number 201-064-4

  •  MDL number MFCD00004679

  •  PubChem Substance ID 24855244

  •  NACRES NA.25

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Properties

Related Categories ACS Grade Buffers, Amino Alcohols, Biochemicals and Reagents, Biological Buffers, Buffers A to Z,
Quality Level   200
grade   ACS reagent
assay   ≥99.8%
  99.8-100.1%
form   crystalline
impurities   ≤0.005% insolubles
  ≤2% water
useful pH range   7 - 9
pKa (25 °C)   8.1
bp   219-220 °C/10 mmHg (lit.)
mp   167-172 °C (lit.)
cation traces   Fe: ≤5 ppm
  heavy metals (as Pb): ≤5 ppm
absorption   passes test
SMILES string   NC(CO)(CO)CO
InChI   1S/C4H11NO3/c5-4(1-6,2-7)3-8/h6-8H,1-3,5H2
InChI key   LENZDBCJOHFCAS-UHFFFAOYSA-N

Description

General description

Tris(hydroxymethyl)aminomethane is mainly required for preparation of buffers at physiological range of 7.3 to 7.5. The prepared buffers are compatible with biological fluids. It is of importance to laboratories as a standard pH solution.

Application

Tris(hydroxymethyl)aminomethane has been used as a buffer solution for lactate dehydrogenase assay, in situ hybridization procedure and protein extraction from cells.

Packaging

100, 500 g in poly bottle

Other Notes

Easily compare specifications for Trizma products with the Trizma specification table.

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 1
RTECS 
TY2900000
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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pHast Pack
Protocols & Articles

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Peer-Reviewed Papers
15

References

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