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27546 Millipore

Clostridial Nutrient Medium

for microbiology

Synonym: RCM, Reinforced Clostridial Medium



Related Categories A - M, All Food Control Media (alphabetical), All Liquid Media/Broths, Anaerobic Bacteria, Bacillus,
grade   for microbiology
shelf life   limited shelf life, expiry date on the label
composition   agar, 0.5 g/L
  L-cysteine hydrochloride, 0.5 g/L
  D(+)-glucose, 5.0 g/L
  meat extract, 10.0 g/L
  peptone, 5.0 g/L
  sodium acetate, 3.0 g/L
  sodium chloride, 5.0 g/L
  starch, 1.0 g/L
  yeast extract, 3.0 g/L
final pH   6.8±0.2 (25 °C)
Featured Industry   Agriculture
Clinical Testing
Food and Beverages
suitability   Anaerobic Bacteria
  nonselective for Bacillus
  nonselective for Clostridia
  nonselective for Escherichia coli and Coliforms
  nonselective for Staphylococcus



For the cultivation and enumeration of clostridia and other anaerobes as well as facultative microorganisms in food, clinical and other material acc. to Hirsch & Grinsted.

Other Notes

Thermal resistance of non-proteolytic type B and type E Clostridium botulinum spores

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 


Certificate of Analysis

Protocols & Articles


Clostridium perfringens, their Properties and their Detection

Clostridia are relatively large, Gram-positive, rod-shaped bacteria that can undergo only anaerobic metabolism. Most Clostridia cannot grow under aerobic conditions and can even be killed by exposure...
AnalytiX Volume 10 Article 4
Keywords: Aerobic, Anaerobic, Chlorinations, Degradations, Environmental, Fermentation, Germination, Gram stain, Indicators, Metabolism, Microbiology

Microbiology Introduction

An initial aim of all microbiologists is the reproducible growth of their microbial cultures, no matter whether the microorganisms are of natural origin or have been genetically engineered by man. Re...
Keywords: Absorption, Aerobic, Anaerobic, Antibiotics, Antimicrobials, Buffers, Culture media, Diffusion, Environmental, Filtration, Genetic, Growth factors, Metabolism, Phase transitions, Precipitation, Reductions, Respiration, Sample preparations, Vitamins

Microbiology Theory: Media Preparation

Microorganisms need nutrients, a source of energy and certain environmental conditions in order to grow and reproduce. In the natural environment, microbes have adapted to the habitats most suitable ...
Keywords: Culture media, Digestions, Environmental, Filtration, Growth factors, Indicators, Microbiology, Phase transitions, Precipitation, Vitamins

Peer-Reviewed Papers


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