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318337 Sigma-Aldrich

Acridine Orange hydrochloride hydrate

≥98% (HPLC)

Synonym: 3,6-Bis(dimethylamino)acridine hydrochloride

  • CAS Number 65-61-2

  • Empirical Formula (Hill Notation) C17H19N3 · HCl · xH2O

  • Molecular Weight 301.81 (anhydrous basis)

  •  Colour Index Number 46005

  •  Beilstein/REAXYS Number 3734978

  •  EC Number 200-614-0

  •  MDL number MFCD00150070

  •  PubChem Substance ID 24859123

  •  NACRES NA.47

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Description

Application

A rigorously purified zinc-free material suitable for use in flow cytometry.

Acridine Orange hydrochloride hydrate dye has been used:
• to stain intracellular acidic vesicles.
• to measure lysosomal membrane permeability (LMP).
• or autophagy detection.

Fluorescent stain for nucleic acids in agarose and polyacrylamide gels. Has been used to study apoptosis in various cell culture and tissue models.

Packaging

1, 5 g in glass bottle

250 mg in glass bottle

Biochem/physiol Actions

Acridine Orange is a metachromatic dye which can stain DNA, RNA and acid glycosaminoglycans. At low concentrations, it intercalates into DNA and precipitates RNA. However, at high concentrations it denatures and precipitates both RNA and DNA. Acridine Orange is also used to analyze autophagy. It enters into acidic organelles in a pH-dependent manner. At neutral pH, acridine orange gives a green fluorescence and in acidic conditions, it accumulates in acidic organelles giving a bright red fluorescence.

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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
RTECS 
AR7601000

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles
Peer-Reviewed Papers
15

References

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