318906 Sigma-Aldrich

Eosin Y solution

5 wt. % in H2O

Synonym: 2′,4′,5′,7′-Tetrabromofluorescein disodium salt, Acid red 87, Eosin yellowish solution

  • CAS Number 17372-87-1

  • Empirical Formula (Hill Notation) C20H6Br4Na2O5

  • Molecular Weight 691.85

  •  Beilstein/REAXYS Number 1443944

  •  MDL number MFCD00005040

  •  PubChem Substance ID 24858876

  •  NACRES NA.47



Related Categories Biochemicals and Reagents, Cell Biology, E-F, Fluorescein and Derivatives, Fluorescent Labels,
Quality Level   100
concentration   5 wt. % in H2O
density   1.015 g/mL at 25 °C
λmax   518 nm
SMILES string   [Na+].[Na+].[O-]C(=O)c1ccccc1C2=C3C=C(Br)C(=O)C(Br)=C3Oc4c(Br)c([O-])c(Br)cc24
InChI   1S/C20H8Br4O5.2Na/c21-11-5-9-13(7-3-1-2-4-8(7)20(27)28)10-6-12(22)17(26)15(24)19(10)29-18(9)14(23)16(11)25;;/h1-6,25H,(H,27,28);;/q;2*+1/p-2



500 mL in glass bottle

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable


Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
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What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Is Product 318906 suitable for use in Hematoxylin and Eosin (H&E) staining procedures?
Product 318906 was not intended for use with H&E staining techniques. The dye content for the Eosin Y is 5% in water.For H&E staining, the concentration is typically 0.5 to 1.0 %. At 5 % dye content, the dye is much too concentrated for use with H&E staining. If used at such a high concentration, the cytoplasmic staining is likely to obscure the nuclear staining from the hematoxylin. A well stained H&E slide should show blue nuclear staining and typically at least 3 shades of Eosin staining. When used at 5% dye content, the cytoplasmic staining is likely to be a homogenous or monotone.The 5% Eosin solution has only one common application - determining sperm viability. In a typical semen analysis, 5% Eosin Y is used in conjunction with 10% Nigrosin. This combination of dyes is used to judge the viability of the sperm. A standard H&E procedure is then used to judge the morphology of the sperm. Sperm counts are typically performed without the use of a dye solution.It is possible to consider a 5% Eosin solution as a 10X staining solution. The product, however, is not suitability tested for use in either a sperm viability procedure or a H&E staining procedure.The following products have been suitability tested for use in H&E staining procedures and are considered suitable for use in In Vitro Diagnostic procedures. They are the preferred products for H&E staining.Most are formulated with 0.5% Eosin Y. The exceptionis Eosin Y with Phloxine B. For this formulation both the Eosin Y and the Phloxine B are formulated at 0.1% dye content.Eosin Y solution, alcoholicHT110116, HT110132,HT110180, HT1101128Eosin Y solution, aqueousHT110216, HT110232, HT110280, HT1102128Eosin Y solution, alcoholic, with phloxineHT110316, HT110332, HT110380, HT1103128
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