322415 Sigma-Aldrich


anhydrous, 99.8%

Synonym: Methyl alcohol

  • CAS Number 67-56-1

  • Linear Formula CH3OH

  • Molecular Weight 32.04

  •  Beilstein Registry Number 1098229

  •  EC Number 200-659-6

  •  MDL number MFCD00004595

  •  PubChem Substance ID 57648672



Related Categories Allium cepa (Onion), Anhydrous, Anhydrous Solvents, Cell Biology, Citrus aurantium (Seville orange),
grade   anhydrous
vapor density   1.11 (vs air)
vapor pressure   410 mmHg ( 50 °C)
  97.68 mmHg ( 20 °C)
assay   99.8%
form   liquid
autoignition temp.   725 °F
expl. lim.   36 %
impurities   <0.002% water
  <0.005% water (100 mL pkg)
evapn. residue   <0.0003%
refractive index   n20/D 1.329(lit.)
bp   64.7 °C(lit.)
mp   −98 °C (lit.)
density   0.791 g/mL at 25 °C(lit.)


Other Notes

Pure-Pac® II containers require the Micromatic MacroValve coupler for dispensing solvents, Z560723.


1, 6×1, 2, 4×2 L in Sure/Seal™

18 L in Pure-Pac™ 1

20 L in Pure-Pac™ 2

View returnable container options.

100, 12×100 mL in Sure/Seal™

200 L in Pure-Pac™ 1


Methanol has been used for fixing the tissues during preparation of thin giemsa stained blood smears. It has been used in the purification step during the synthesis of pyocyanin, a toxic compound found in Pseudomonas aeruginosa.

Methanol has been used in following applications:
• Used for the synthesis and purification of pyocyanin, spectrophotometric estimation of pyocyanin concentration, and the calculation of pyocyanin molar concentration using spectrophotometer absorption data.
• Used as a component of staining solution used in karyotyping.
• Used as a component of staining solution to stain cell colonies in colony formation assay.

General description

Methanol is an industrial organic solvent commonly used in organic synthesis. It is being considered as a potential hydrogen source in fuel cell technology due to its high H/C ratio, low propensity for soot generation, relatively low reforming temperature and its liquid state at room temperature. The olefins (ethylene or propylene) formed from methanol via MTO (methanol-to-olefins) process, can be an alternative to oil and gas to produce hydrocarbon fuels.

Legal Information

Pure-Pac is a registered trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

Signal word 
Hazard statements 
UN1230 - class 3 - PG 2 - Methanol
WGK Germany 
Flash Point(F) 
48.2 °F
Flash Point(C) 
9 °C


Certificate of Analysis

Certificate of Origin

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Protocols & Articles


Cross Coupling Reactions of Chiral Secondary Organoboronic Esters With Retention of Configuration-

We describe a systematic study of the scope and relationship between ligand structure and activity for a highly efficient and selective class of catalysts containing sterically hindered chelating alk...
Keywords: Aminations, Gas chromatography, Ligands

Mild and general palladium-catalyzed synthesis of methyl aryl ethers enabled by the use of a palladacycle precatalyst

A general method for the Pd-catalyzed coupling of methanol with (hetero)aryl halides is described. The reactions proceed under mild conditions with a wide range of aryl and heteroaryl halides to give...
Keywords: Catalysis, Chromatography, Column chromatography

Pressure-Temperature Calculator for Solvents

*Calculations provide estimated results based on Antoine's Coefficients and their associated temperature range values. Antoine's Coefficients were obtained from the National Institute of Standards an...

Related Content

Pressure-Temperature Nomograph Interactive Tool

The Pressure-Temperature Nomograph tool is an application of the Clausius-Clapeyron Equation, which assumes the heat of vaporization is a constant over a pressure range. Antoine´s Equation gets aroun...
Keywords: Evaporation, Vaporization

Properties of Solvents Table

a. Gant, R., LC/GC 10 (7), 515 (1992) 1. At 20 °C 2. At 20 °C +/- 5 °C relative to water at 4 °C 3. At 10 mmHg pressure 4. At l=589.32 nm
Keywords: Gas chromatography, High performance liquid chromatography

Peer-Reviewed Papers


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