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34046 Supelco

Lysozyme from chicken egg white

VETRANAL, analytical standard

Synonym: Mucopeptide N-acetylmuramoylhydrolase, Muramidase

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Properties

Related Categories 3.2.x.x Glycosidases, 3.x.x.x Hydrolases, Amino Acid, Peptide & Protein Standards, Analytical Standards, Analytical/Chromatography,
Quality Level   100
grade   analytical standard
product line   VETRANAL
form   neat
shelf life   limited shelf life, expiry date on the label
mol wt   single-chain 14.3 kDa
  Mr ~14000
application(s)   HPLC: suitable
  gas chromatography (GC): suitable
Featured Industry   Food and Beverages
analyte suitability   amino acids
  peptides
  proteins
format   neat
storage temp.   2-8°C

Description

General description

Lysozyme from chicken egg white, is a protein that can show broad spectrum of antibacterial activity against gram positive and gram negative bacteria. Its antimicrobial activity can be enhanced by pretreatment of the target microbial cells with chelating agents like ethylenediaminetetraacetic acid (EDTA).

Lysozyme from chicken egg white, is a protein that can show broad spectrum of antibacterial activity against gram positive and gram negative bacteria. Its antimicrobial activity can be enhanced by pretreatment of the target microbial cells with chelating agents like ethylenediaminetetraacetic acid (EDTA).

Application

Lysozyme from chicken egg white may be used as an analytical reference standard for the determination of the analyte in:
• Hard and pasta filata cheeses using reversed-phase high performance liquid chromatography with diode array and fluorimetric detection [(RP-HPLC-DAD) and (RP-HPLC-FLD)].
• Food samples using high-performance liquid chromatography (HPLC) and enzyme-linked immunosorbent assay (ELISA).

Enzyme breaks down the cell walls of bacteria; used to prepare spheroplasts.

Biochem/physiol Actions

Lysozyme hydrolyzes β(1→4) linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrin. Gram-positive cells are quite susceptible to this hydrolysis as their cell walls have a high proportion of peptidoglycan. Gram-negative bacteria are less susceptible due to the presence of an outer membrane and a lower proportion of peptidoglycan. However, these cells may be hydrolyzed in the presence of EDTA that chelates metal ions in the outer bacterial membrane.

The enzyme is active over a broad pH range (6.0 to 9.0). At pH 6.2, maximal activity is observed over a wider range of ionic strengths (0.02 to 0.100 M) than at pH 9.2 (0.01 to 0.06 M).

Legal Information

VETRANAL is a trademark of Sigma-Aldrich Chemie GmbH

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

HPLC Analysis Proteins on Proteomix® SCX-NP1.7, Demonstration of Column Stability with Repeated Injections

From our library of Articles, Sigma-Aldrich presents HPLC Analysis Proteins on Proteomix® SCX-NP1.7, Demonstration of Column Stability with Repeated Injections
Keywords: Chromatography, High performance liquid chromatography

HPLC Analysis of Four Low Moleculer Weight Proteins on 5μm BIOshell™ 160Å Peptide C18 Column, Comparison to Totally Porous 3 μm C18 Column

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Four Low Moleculer Weight Proteins on 5μm BIOshell™ 160Å Peptide C18 Column, Comparison to Totally Porous 3 μm C18 Column
Keywords: Chromatography, High performance liquid chromatography

HPLC Analysis of Lysozyme on SRT® SEC-300, 30 cm x 7.8 mm I.D., 5 μm particles

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Lysozyme on SRT® SEC-300, 30 cm x 7.8 mm I.D., 5 μm particles
Keywords: Chromatography, High performance liquid chromatography, Size-exclusion chromatography

HPLC Analysis of Peptides and Proteins on Proteomix® SCX-NP1.7, 5 cm x 4.6 mm I.D. Column

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Peptides and Proteins on Proteomix® SCX-NP1.7, 5 cm x 4.6 mm I.D. Column
Keywords: Chromatography, High performance liquid chromatography

HPLC Analysis of Protein Standards (1) on BIOshell™ A400 Protein C4

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Protein Standards (1) on BIOshell™ A400 Protein C4
Keywords: Chromatography, High performance liquid chromatography, Reversed-phase chromatography

HPLC Analysis of Proteins Comparing Ion Selectivity on Proteomix® SCX-NP5

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Proteins Comparing Ion Selectivity on Proteomix® SCX-NP5
Keywords: Chromatography, High performance liquid chromatography, Ion Exchange

HPLC Analysis of Proteins Comparing Ion Selectivity on Proteomix® WCX-NP5

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Proteins Comparing Ion Selectivity on Proteomix® WCX-NP5
Keywords: Chromatography, High performance liquid chromatography, Ion Exchange

HPLC Analysis of Proteins Demonstrating Selectivity Differences between TSKgel® Strong (SP-5PW) and Weak (CM-5PW) Cation Exchangers

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Proteins Demonstrating Selectivity Differences between TSKgel® Strong (SP-5PW) and Weak (CM-5PW) Cation Exchangers
Keywords: Chromatography, High performance liquid chromatography

HPLC Analysis of Proteins and Peptides on Proteomix® WCX, Comparison of Different Particle Sizes

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Proteins and Peptides on Proteomix® WCX, Comparison of Different Particle Sizes
Keywords: Chromatography, High performance liquid chromatography

HPLC Analysis of Proteins and Peptides on Proteomix® WCX-NP1.7 Demonstrating Lot-to-Lot Reproducibility

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Proteins and Peptides on Proteomix® WCX-NP1.7 Demonstrating Lot-to-Lot Reproducibility
Keywords: Chromatography, High performance liquid chromatography

HPLC Analysis of Proteins by Cation Exchange on Proteomix® WCX-NP5: Affect of pH

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Proteins by Cation Exchange on Proteomix® WCX-NP5: Affect of pH
Keywords: Chromatography, High performance liquid chromatography, Ion Exchange

HPLC Analysis of Proteins by Cation Exchange on Proteomix® WCX: Affect of Particle Size

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Proteins by Cation Exchange on Proteomix® WCX: Affect of Particle Size
Keywords: Chromatography, High performance liquid chromatography, Ion Exchange

HPLC Analysis of Proteins on Antibodix® WCX-NP10, Comparison of WCX Columns

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Proteins on Antibodix® WCX-NP10, Comparison of WCX Columns
Keywords: Chromatography, High performance liquid chromatography

HPLC Analysis of Proteins on BIOshell™ A160 Peptide C18

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Proteins on BIOshell™ A160 Peptide C18
Keywords: Chromatography, High performance liquid chromatography

HPLC Analysis of Proteins on BIOshell™ A400 Protein C18

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Proteins on BIOshell™ A400 Protein C18
Keywords: High performance liquid chromatography

HPLC Analysis of Proteins on Proteomix® SCX Columns, Effect of Particle Size of Resolution

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Proteins on Proteomix® SCX Columns, Effect of Particle Size of Resolution
Keywords: Chromatography, High performance liquid chromatography

HPLC Analysis of Proteins on Proteomix® SCX-NP1.7, Demonstration of Lot-to-Lot Reproducibility

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Proteins on Proteomix® SCX-NP1.7, Demonstration of Lot-to-Lot Reproducibility
Keywords: Chromatography, High performance liquid chromatography

HPLC Analysis of Proteins on Proteomix® SCX-NP5

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Proteins on Proteomix® SCX-NP5
Keywords: High performance liquid chromatography, Separation

HPLC Analysis of Proteins on Proteomix® WCX-NP5

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Proteins on Proteomix® WCX-NP5
Keywords: High performance liquid chromatography, Proteomics

HPLC Analysis of Proteins on TSKgel® BioAssist S, Ion Selectivity Comparison

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Proteins on TSKgel® BioAssist S, Ion Selectivity Comparison
Keywords: Chromatography, High performance liquid chromatography, Ion Exchange

HPLC Analysis of Proteins on TSKgel® BioAssist S

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Proteins on TSKgel® BioAssist S
Keywords: High performance liquid chromatography, Proteomics

HPLC Analysis of Proteins on TSKgel® Butyl-NPR

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Proteins on TSKgel® Butyl-NPR
Keywords: Chromatography, High performance liquid chromatography

HPLC Analysis of Proteins on TSKgel® Phenyl-5PW

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Proteins on TSKgel® Phenyl-5PW
Keywords: Chromatography, High performance liquid chromatography

HPLC Analysis of Proteins on TSKgel® Porous (Phenyl-5PW and Ether-5PW) and Non-Porous (Butyl-NPR) HIC Columns

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Proteins on TSKgel® Porous (Phenyl-5PW and Ether-5PW) and Non-Porous (Butyl-NPR) HIC Columns
Keywords: Chromatography, High performance liquid chromatography

HPLC Analysis of Proteins with Different pI Values on Proteomix® SCX-NP3

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Proteins with Different pI Values on Proteomix® SCX-NP3
Keywords: Chromatography, High performance liquid chromatography

Scalability of TSKgel® Phenyl-5PW Analytical HPLC Separations to Preparative/Process Scale on Toyopearl® Phenyl-650M Resins

From our library of Articles, Sigma-Aldrich presents Scalability of TSKgel® Phenyl-5PW Analytical HPLC Separations to Preparative/Process Scale on Toyopearl® Phenyl-650M Resins
Keywords: Chromatography, High performance liquid chromatography

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Peer-Reviewed Papers
15

References

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