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43291 Millipore

m-FC Agar, Vegitone

for microbiology, NutriSelect Plus

Synonym: Membrane Fecal Coliform Agar, Vegitone, Vegitone m-FC Agar, m-Fecal Coliform Agar, Vegitone

  •  NACRES NA.85

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Properties

Related Categories A - M, All Food Control Media (alphabetical), All Solid Media/Agars, Escherichia coli & Coliforms, Media by Application,
Quality Level   100
grade   for microbiology
form   powder
shelf life   limited shelf life, expiry date on the label
composition   agar, 15.0 g/L
  anilne blue, 0.1 g/L
  lactose, 12.5 g/L
  proteose peptone (vegetable), 5.0 g/L
  sodium chloride, 5.0 g/L
  synthetic detergent, 1.5 g/L
  tryptose (vegetable), 10.0 g/L
  yeast extract, 3.0 g/L
mfr. no.   NutriSelect Plus
application(s)   bioburden testing: suitable
  microbiological culture: suitable
  water monitoring: suitable
final pH   7.4±0.2 (25 °C)
Featured Industry   Agriculture
Environmental
Food and Beverages
suitability   selective and differential for Escherichia coli
  selective and differential for Salmonella spp.
  selective and differential for Shigella spp.
  selective and differential for coliforms

Description

Application

M-FC Agar is used for the detection and enumeration of faecal coliforms using membrane filter technique at higher temperature. In this modified medium the animal derived peptones are replaced by plant peptones.

Preparation Note

Dissolve 52.1 g in 1 litre distilled water and heat to boiling to dissolve completely. Add 10 ml of a 1 % solution of rosolic acid (Cat. No. 83910) in 0.2 N NaOH. Continue heating for 1 minute with frequent agitation. Do not autoclave! Cool to 45-50°C. Dispense 4 ml amounts into Petri dishes (50-60mm) and allow to solidify.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Microbiology Introduction

An initial aim of all microbiologists is the reproducible growth of their microbial cultures, no matter whether the microorganisms are of natural origin or have been genetically engineered by man. Re...
Keywords: Absorption, Aerobic, Anaerobic, Antibiotics, Antimicrobials, Buffers, Culture media, Diffusion, Environmental, Filtration, Genetic, Growth factors, Metabolism, Phase transitions, Precipitation, Reductions, Respiration, Sample preparations, Vitamins

Microbiology Theory: Media Preparation

Microorganisms need nutrients, a source of energy and certain environmental conditions in order to grow and reproduce. In the natural environment, microbes have adapted to the habitats most suitable ...
Keywords: Culture media, Digestions, Environmental, Filtration, Growth factors, Indicators, Microbiology, Phase transitions, Precipitation, Vitamins

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