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439142 Supelco

Chloroform

for HPLC, ≥99.8%, contains 0.5-1.0% ethanol as stabilizer

Synonym: Methylidyne trichloride, Trichloromethane

  • CAS Number 67-66-3

  • Empirical Formula (Hill Notation) CHCl3

  • Molecular Weight 119.38

  •  Beilstein/REAXYS Number 1731042

  •  MDL number MFCD00000826

  •  PubChem Substance ID 24867327

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Properties

Related Categories Analytical Reagents, Analytical/Chromatography, Chloroform, Chromatography Reagents & Solvents, HPLC Grade Solvents,
Quality Level   100
grade   for HPLC
vapor density   4.1 (vs air)
vapor pressure   160 mmHg ( 20 °C)
assay   ≥99.8%
contains   0.5-1.0% ethanol as stabilizer
packaging   PVC-coated bottle of 4, 4x4 L
impurities   <0.01% water
evapn. residue   <0.0003%
color   APHA: ≤10
refractive index   n20/D 1.445 (lit.)
bp   60.5-61.5 °C (lit.)
mp   −63 °C (lit.)
density   1.48 g/mL at 25 °C
  1.492 g/mL at 25 °C (lit.)
cation traces   Pb: ≤0.05 ppm
λ   H2O reference
UV absorption   λ: 245 nm Amax: 1.00
  λ: 255 nm Amax: 0.15
  λ: 260 nm Amax: 0.15
  λ: 270 nm Amax: 0.02
  λ: 290-400 nm Amax: 0.01
Featured Industry   Food and Beverages
SMILES string   ClC(Cl)Cl
InChI   1S/CHCl3/c2-1(3)4/h1H
InChI key   HEDRZPFGACZZDS-UHFFFAOYSA-N

Description

General description

Chloroform is a colorless, volatile chlorinated organic solvent whose vapors have a narcotic effect. It is susceptible to degradation with time, which can be suppressed by adding ethanol as a stabilizer. Since ethanol is added in higher concentration it leads to increase in polarity which may have a potential influence on some of the applications. Reports suggest that chloroform stabilized with ethanol reduces chances on creating artifacts during the chromatographic separation after extraction of anthracyclines from biological matrices.

Application

Chloroform was used as an organic phase in the methylene blue (MB) two-phase titration process for estimating the cationic surfactant content in high salinity brine.
It may be used as:
• Solvent for electropolymerization.
• Solvent for recrystallization.
• Extractant in solvent extraction process.

Packaging

4, 4×4 L in PVC-coated bottle

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Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Target organs 
Central nervous system
RIDADR 
UN 1888 6.1 / PGIII
WGK Germany 
WGK 3
Flash Point(F) 
does not flash
Flash Point(C) 
does not flash

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

Articles

Pressure-Temperature Calculator for Solvents

*Calculations provide estimated results based on Antoine's Coefficients and their associated temperature range values. Antoine's Coefficients were obtained from the National Institute of Standards an...

Peer-Reviewed Papers
15

References

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