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Spectinomycin dihydrochloride pentahydrate

VETRANAL, analytical standard

  • CAS Number 22189-32-8

  • Empirical Formula (Hill Notation) C14H24N2O7 · 2HCl · 5H2O

  • Molecular Weight 495.35

  •  Beilstein/REAXYS Number 5684150

  •  EC Number 244-554-3

  •  MDL number MFCD00150886

  •  PubChem Substance ID 329757229

  •  NACRES NA.24

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Properties

Related Categories Additional Standards, Aminoglycosides, Analytical Standards, Analytical/Chromatography, Antibacterial,
Quality Level   100
grade   analytical standard
InChI Key   DCHJOVNPPSBWHK-UXXUFHFZSA-N
product line   VETRANAL
form   neat
shelf life   limited shelf life, expiry date on the label
application(s)   HPLC: suitable
  gas chromatography (GC): suitable
solubility   H2O: soluble at 
Featured Industry   Pharmaceutical (small molecule)
Mode of action   protein synthesis | interferes
antibiotic activity spectrum   Gram-negative bacteria
  Gram-positive bacteria
format   neat
storage temp.   2-8°C

Description

General description

Chemical structure: aminoglycoside

Application

Refer to the product′s Certificate of Analysis for more information on a suitable instrument technique. Contact Technical Service for further support.

Spectinomycin dihydrochloride pentahydrate may be used as a reference standard for the determination of spectinomycin in bovine milk samples by liquid chromatography with electrochemical detection.

Spectinomycin is an aminocyclitol antibiotic derived from Streptomyces spectabilis. It has been shown to have a wide variety of uses, including treating acute gonorrheal urethritis and cervictis, to mark cell layers to monitor cell fate during leaf development, as a selection marker in plant related transformation systems for plant cells containing the marker gene Spcr, to study respiratory tract infections of cattle caused by Pasteurella multocida and Mannheimia haemolytica, and to generate plants deficient for the plastid-encoded RNA polymerase on MS-agar media. Spectinomycin also has use in amplification of low copy number plasmid carrying replicons. It is recommended for use in cell culture applications at 7.5-20 mg/L.

Caution

Solutions can be stored at 2-8°C for several weeks or at -20°C for more extended periods.

Preparation Note

Stock solutions should be prepared at 10 mg/mL in water and filter sterilized.

Legal Information

VETRANAL is a trademark of Sigma-Aldrich Chemie GmbH

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2

Documents

Certificate of Analysis (COA)

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Protocols & Articles
Peer-Reviewed Papers
15

References

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