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50553 Sigma-Aldrich

α-tri-Calcium phosphate

Reagent for transient & stable DNA transfections

Synonym: α-TCP, α-Tricalcium phosphate

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Properties

Related Categories Analytical Standards, Analytical/Chromatography, Calcium, Chemical Synthesis, Inorganic Salts,
Quality Level   100
grade   for molecular biology
form   powder
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storage temp.   room temp
SMILES string   [Ca++].[Ca++].[Ca++].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O
InChI   1S/3Ca.2H3O4P/c;;;2*1-5(2,3)4/h;;;2*(H3,1,2,3,4)/q3*+2;;/p-6
InChI key   QORWJWZARLRLPR-UHFFFAOYSA-H

Description

General description

α-tri-Calcium is a tasteless powder containing excess of calcium oxide. It is commonly used to introduce DNA and transfect eukaryotic cells for molecular biology experiments.

α-tri-Calcium phosphate (α-TCP) can be made by heating the low-temperature polymorph β-TCP. It can also be made by thermal crystallization of amorphous precursors with the appropriate composition above the transformation temperature. α-TCP is biocompatible as β-TCP. It is highly soluble and hydrolyses quickly to calcium-deficient hydroxyapatite. This makes α-TCP a beneficial constituent for formulating self-setting osteotransductive bone cements and biodegradable bioceramics and composites for bone repairing.

Application

For analysis of bone cement; as standard.
α-tri-Calcium phosphate has been used to transfect 293T and 231 cells with the PiggyBac LSS-mOrange- aspartate-glutamate-valine-aspartate (DEVD)-mKate2 transposon vector and in reporter verification studies.

Suitable for
• transient and stable transfection of wide range of cell types
• drug delivery systems

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Protocols & Articles
Peer-Reviewed Papers
15

References

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