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51489 Millipore

Rapid Coliform ChromoSelect Broth

suitable for microbiology, NutriSelect Plus

Synonym: Coliform Rapid ChromoSelect Broth

  •  NACRES NA.85

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Properties

Related Categories A - M, All Food Control Media (alphabetical), All Liquid Media/Broths, Chromogenic Media, Escherichia coli & Coliforms,
Quality Level   100
form   powder
shelf life   limited shelf life, expiry date on the label
composition   chromogenic substrate, 0.08 g/L
  dipotassium hydrogen phosphate, 2.7 g/L
  fluorogenic substrate, 0.05 g/L
  IPTG, 0.1 g/L
  peptone, special, 5 g/L
  potassium dihydrogen phosphate, 2 g/L
  sodium chloride, 5 g/L
  sodium lauryl sulfate, 0.1 g/L
  sorbitol, 1 g/L
mfr. no.   NutriSelect Plus
application(s)   bioburden testing: suitable
  microbiology: suitable (microbial identification)
  microbiology: suitable
  water monitoring: suitable
final pH   6.8±0.3 (25 °C)
Featured Industry   Agriculture
Environmental
Food and Beverages
storage temp.   2-8°C
suitability   selective and differential for Escherichia coli
  selective and differential for coliforms

Description

Application

Rapid Coliform ChromoSelect Broth is used for detection and conformation of Escherichia coli and coliforms on the basics of enzyme substrate reaction from water samples, using a combination of chromogenic and fluorogenic substrate.

Preparation Note

Suspend 16.0 g (single strength) or 32.0 g (double strength) in 1 litre distilled water. Boil to dissolve the medium completely. Dispense as desired and sterilize by autoclaving at 1 bar pressure (121 °C) for 15 minutes.

Other Notes

Medium for the assay of water for Coliforms

Legal Information

NutriSelect is a trademark of Merck KGaA, Darmstadt, Germany

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Microbiology Introduction

An initial aim of all microbiologists is the reproducible growth of their microbial cultures, no matter whether the microorganisms are of natural origin or have been genetically engineered by man. Re...
Keywords: Absorption, Aerobic, Anaerobic, Antibiotics, Antimicrobials, Buffers, Culture media, Diffusion, Environmental, Filtration, Genetic, Growth factors, Metabolism, Phase transitions, Precipitation, Reductions, Respiration, Sample preparations, Vitamins

Microbiology Theory: Media Preparation

Microorganisms need nutrients, a source of energy and certain environmental conditions in order to grow and reproduce. In the natural environment, microbes have adapted to the habitats most suitable ...
Keywords: Culture media, Digestions, Environmental, Filtration, Growth factors, Indicators, Microbiology, Phase transitions, Precipitation, Vitamins

Overview of Chromogenic Media

The use of traditional versus improved media formulation containing chromogenic substrate is currently an important topic in the field of microbiology. The focus behind such developments was to produ...
Jvo SiegristProduct Manager
AnalytiX Issue 3
Keywords: Culture media, Diffusion, Electron capture detector, Fermentation, Filtration, Metabolites, Microbiology

Peer-Reviewed Papers
15

References

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