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51558 Sigma-Aldrich

HEPES buffered saline

BioUltra, for molecular biology, 2× concentrate

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Properties

Related Categories Alphabetical, BioUltra, BioUltra Buffers, Biochemicals and Reagents, Biological Buffers,
Quality Level   200
grade   for molecular biology
product line   BioUltra
form   liquid
quality   2× concentrate
composition   dextrose, 2.0 g/L
  HEPES, 10 g/L
  KCl, 0.74 g/L
  NaCl, 16 g/L
  Na2HPO4.2H2O, 0.27 g/L
concentration   2 ×
impurities   DNases, none detected
  RNases, none detected
  phosphatases, none detected
  proteases, none detected
refractive index   n20/D 1.339
pH   7.2 (25 °C)
cation traces   Al: ≤1 mg/kg
  Ba: ≤1 mg/kg
  Bi: ≤1 mg/kg
  Ca: ≤5 mg/kg
  Cd: ≤1 mg/kg
  Co: ≤1 mg/kg
  Cr: ≤1 mg/kg
  Cu: ≤1 mg/kg
  Fe: ≤1 mg/kg
  Li: ≤1 mg/kg
  Mg: ≤1 mg/kg
  Mn: ≤1 mg/kg
  Mo: ≤1 mg/kg
  Ni: ≤1 mg/kg
  Pb: ≤1 mg/kg
  Sr: ≤1 mg/kg
  Zn: ≤1 mg/kg
λ   neat
UV absorption   λ: 260 nm Amax: 0.06
  λ: 280 nm Amax: 0.04
SMILES string   OCCN1CCN(CC1)CCS(O)(=O)=O
InChI   1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)
InChI key   JKMHFZQWWAIEOD-UHFFFAOYSA-N

Description

General description

HEPES buffered saline is a tissue culture buffer, which is used to maintain the pH of cell culture. It is water soluble and atmosphere independent. HEPES destroys magnesium in sodium chloride solutions.

Application

HEPES buffered saline has been used:
• for the preparation of plasmid DNA
• as a reagent to prepare transfection master mix for DNA construct vectors
• to develop hydrogel precursor for cell leakage assay

Components

concentration (in H2O): 10 g/l HEPES; 16 g/l NaCl; 0.74 g/l KCl; 0.27 g/l Na2HPO4.2H2O; 2.0 g/l dextrose

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 1
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Protocols & Articles

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Peer-Reviewed Papers
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References

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