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54461 Sigma-Aldrich

HEPES

≥99.0% (T)

Synonym: 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)

  • CAS Number 7365-45-9

  • Empirical Formula (Hill Notation) C8H18N2O4S

  • Molecular Weight 238.30

  •  Beilstein/REAXYS Number 883043

  •  EC Number 230-907-9

  •  MDL number MFCD00006158

  •  PubChem Substance ID 329758032

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Properties

assay   ≥99.0% (T)
form   powder or crystals
ign. residue   ≤0.1% (as SO4)
loss   ≤0.5% loss on drying, 110 °C
pH   5.0-6.5 (25 °C, 238 g/L)
useful pH range   6.8 - 8.2
pKa (25 °C)   7.5
solubility   water: 2.38 g/10 mL, clear to slightly hazy, colorless to almost colorless
anion traces   chloride (Cl-): ≤50 mg/kg
  sulfate (SO42-): ≤300 mg/kg
cation traces   Ca: ≤50 mg/kg
  Cd: ≤5 mg/kg
  Co: ≤5 mg/kg
  Cr: ≤5 mg/kg
  Cu: ≤5 mg/kg
  Fe: ≤5 mg/kg
  K: ≤50 mg/kg
  Mg: ≤100 mg/kg
  Mn: ≤5 mg/kg
  Na: ≤50 mg/kg
  Ni: ≤5 mg/kg
  Pb: ≤5 mg/kg
  Zn: ≤5 mg/kg
SMILES string   OCCN1CCN(CC1)CCS(O)(=O)=O
InChI   1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)
InChI key   JKMHFZQWWAIEOD-UHFFFAOYSA-N

Description

Other Notes

Biological buffer

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 1
RTECS 
TL6809000
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Protocols & Articles
Peer-Reviewed Papers
15

References

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Description

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H7523 HEPES, BioXtra, pH 5.0-6.5 (1 M in H2O), ≥99.5% (titration)

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