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63163 Millipore

Malachite Green Broth

suitable for microbiology, NutriSelect® Basic



Related Categories A - M, All Liquid Media/Broths, Media by Application, Media by Microorganism, Media for Drinking Water,
Quality Level   100
form   powder
shelf life   limited shelf life, expiry date on the label
composition   dipotassium hydrogen phosphate, 1.1 g/L
  malachite green oxalate, 0.03 g/L
  meat extract, 9 g/L
  meat peptone, 15 g/L
mfr. no.   NutriSelect® Basic
application(s)   bioburden testing: suitable
  microbiological culture: suitable
  microbiology: suitable
  pathogen testing: suitable
  sample preparation: suitable
final pH   7.0±0.2 (25 °C)
Featured Industry   Environmental
Food and Beverages
storage temp.   2-8°C
suitability   selective for Pseudomonas spp.


General description

Malachite Green Broth is for the selective enrichment of Pseudomonas aeruginosa according to Habs and Kirschner.
The culture medium has been recommended by Schubert and Blum for the examination of ground-, surface-, drinking-, bathing and process-water. In addition, it is suitable for the examination of mineral and spring-water.


For the selective enrichment of Pseudomonas aeruginosa according to Habs and Kirschner.

Malachite Green Broth has been used for the growth of Pseudomonas aeruginosa from water samples.

Preparation Note

Dissolve 25 g in 1 litre distilled water. Pour into small containers and autoclave at 121°C for 15 minutes.

Legal Information

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety & Documentation

Safety Information

Personal Protective Equipment 
NONH for all modes of transport
WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable


Certificate of Analysis (COA)

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Protocols & Articles


Microbiology Introduction

An initial aim of all microbiologists is the reproducible growth of their microbial cultures, no matter whether the microorganisms are of natural origin or have been genetically engineered by man. Re...
Keywords: Absorption, Aerobic, Anaerobic, Antibiotics, Antimicrobials, Buffers, Culture media, Diffusion, Environmental, Filtration, Genetic, Growth factors, Metabolism, Phase transitions, Precipitation, Reductions, Respiration, Sample preparations, Vitamins

Microbiology Theory: Media Preparation

Microorganisms need nutrients, a source of energy and certain environmental conditions in order to grow and reproduce. In the natural environment, microbes have adapted to the habitats most suitable ...
Keywords: Culture media, Digestions, Environmental, Filtration, Growth factors, Indicators, Microbiology, Phase transitions, Precipitation, Vitamins

Pseudomonas Media and Tests

Pseudomonas are motile (one or more polar flagella), rod shaped and aerobe Gram-negative bacteria. They are found almost everywhere, in soil, water, plants and animals. In most cases it is not pathog...
Jvo Siegrist, Product Manager Microbiology, ivo.siegrist@sial.com
Analytix Volume 2007 Article 5
Keywords: Anaerobic, Antifungals, Biochemistry, Cosmetics, Diagnostic, Environmental, Fermentation, Glycolysis, Metabolism, Metabolites, Microbiology, Pentose phosphate pathway, Pharmaceutical, Respiratory

Peer-Reviewed Papers


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