650609 Sigma-Aldrich


HPLC Plus, ≥99.9%, poly-coated bottles

Synonym: Methyl alcohol

  • CAS Number 67-56-1

  • Linear Formula CH3OH

  • Molecular Weight 32.04

  •  Beilstein Registry Number 1098229

  •  EC Number 200-659-6

  •  MDL number MFCD00004595

  •  PubChem Substance ID 57652016



Related Categories Analytical Reagents, Analytical/Chromatography, Chromatography Reagents & Solvents, HPLC Plus, HPLC Plus Grade Solvents,
grade   HPLC Plus
vapor density   1.11 (vs air)
vapor pressure   410 mmHg ( 50 °C)
  97.68 mmHg ( 20 °C)
assay   ≥99.9%
form   liquid
autoignition temp.   725 °F
expl. lim.   36 %
impurities   ≤0.0002 meq/g Titr. base
  ≤0.0003 meq/g Titr. acid
  ≤0.001% Carbonyl Compounds
  ≤1.0 ppb Fluorescence (quinine) at 254 nm
  ≤1.0 ppb Fluorescence (quinine) at 365 nm
  <0.05% water
evapn. residue   ≤0.0001%
color   APHA: ≤10
refractive index   n20/D 1.329(lit.)
bp   64.7 °C(lit.)
mp   −98 °C (lit.)
solubility   benzene: miscible(lit.)
  ethanol: miscible(lit.)
  water: miscible(lit.)
density   0.791 g/mL at 25 °C(lit.)
  0.791 g/mL at 25 °C
absorption   HPLC-gradient/230 nm ≤2 mAU
  HPLC-gradient/254 nm ≤5 mAU
λ   H2O reference
UV absorption   λ: 205 nm Amax: 1.0
  λ: 210 nm Amax: 0.80
  λ: 220 nm Amax: 0.20
  λ: 230 nm Amax: 0.10
  λ: 250 nm Amax: 0.02
  λ: 400 nm Amax: 0.005



4, 4×4 L in PVC-coated bottle

Preparation Note

Product filtered through a 0.2 μm filter


Methanol (MeOH) may be used in the following studies:
• Colony forming unit-fibroblast assay of bone marrow mononuclear cells.
• As solvent for the preparation of extracts of hyphae of Aspergillus for the estimation of gliotoxin by reversed phase-HPLC.
• Immunofluorescence studies.
• To compose eluent for the ion-pair reverse-phase HPLC isolation of nucleotides and their decomposition products.
• As eluent in the HPLC estimation of malondialdehyde in plasma, which is an indicator of oxidative stress.

Suitable for HPLC, spectrophotometry, and some LC-MS applications

General description

Methanol (MeOH) is an organic solvent with a wide range of industrial applications. It is the main component of lithium batteries. It can be prepared by hydrogenation of CO2 under various conditions. MeOH is a toxic alcohol employed for denaturing ethyl alcohol. A study reports the preparation of biodiesel from canola oil using methanol under supercritical conditions.

Methanol is an alcohol. Its thermochemical conversion to C2-C10 hydrocarbons in the presence of shape-selective zeolites has been reported. Its oxidation on Ru-Pt catalyst system by ruthenium ad-atoms has been proposed.

Safety & Documentation

Safety Information

Signal word 
Hazard statements 
Precautionary statements 
UN1230 - class 3 - PG 2 - Methanol
WGK Germany 
Flash Point(F) 
48.2 °F
Flash Point(C) 
9 °C


Certificate of Analysis

Certificate of Origin

PTFE Millex filter
Protocols & Articles


Pressure-Temperature Calculator for Solvents

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