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66008BC Millipore

SimPlate® Total Coliform & E. coli CI

BioControl, Quantitative results for Coliforms and E. coli from food and environmental samples in just 24 hours

Synonym: CEC-CI (Color Indicator)



material   plastic vials (containing dehydrated culture media)
Agency/Method   AOAC® OMA
form   dry powder
shelf life   6‑18 mo.
mfr. no.   BioControl Systems, 66008-100
  BioControl Systems, 66008-500
storage condition   dry media
Featured Industry   Food and Beverages
compatibility   for use with SimPlate (65009BC)
storage temp.   room temp
suitability   coliforms: Escherichia coli


General description

SimPlate for Total Coliform & E. coli Color Indicator (CEc-CI) method is used for the detection and quantification of the Total Coliform and E. coli populations. It is based on Binary Detection Technology (BDT) which equates the presence of Total Coliform to the presence of a color change in a medium and E. coli is detected by the presence of a blue fluorescent color change in the medium due to the action of the enzyme and Β-glucuronidase. The medium/sample mixture is dispensed into a SimPlate device, and incubated for 24-28 h. The Total Coliform and E. coli count is determined by counting the wells with changed color and referring to the SimPlate Conversion Table. Colored wells indicate the presence of Total Coliforms, and colored wells that fluoresce indicate the presence of E. coli. The SimPlate device is packaged separately.


AOAC Official Method 2005.03

Features and Benefits

•  Total Coliform and E. coli results are available from a single plate. Proprietary chromogenic media and patented plating device with isolation wells combine to produce easily counted results. Because results are not dependent on gas formation, results are more easily interpreted than tube or film-based methods.
•  While other plating methods require duplicate plating of samples, and the standard MPN method requires multiple dilutions and at least nine tubes of media, SimPlate has been validated to provide equivalent results with just a single plate. Combined with the reduction of necessary sample dilutions, made possible by the broad counting range of the plate (1-738), SimPlate reduces the volume of media and number of tests required for each sample, helping to reduce overall testing costs.


2 packages of 50 single-test vials (66008-100), or 1 package of 50 multi-test (10 tests/vial) vials (66008-500)

Testing Method

Binary Detection Technology

Other Notes

Required for use: SimPlate Device (65009BC)

Legal Information

AOAC is a registered trademark of AOAC International

Binary Detection Technology is a trademark of BioControl Systems, Inc.

SimPlate is a registered trademark of BioControl Systems, Inc.

Kit component also available separately


Product #

Add to Cart

SimPlate Coliform & E. coli-Color Indicator (CEc-CI) Single Dose Fill, 100 test    
SimPlate Coliform & E. coli-Color Indicator (CEc-CI) Multi-Dose Fill, 500 test    
Safety & Documentation

Safety Information

NONH for all modes of transport


Certificate of Analysis (COA)

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Protocols & Articles


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