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67309 Millipore

Kovac’s reagent for indoles

for microbiology

Synonym: 4-(Dimethylamino)benzaldehyde solution

  • CAS Number 100-10-7

  • Empirical Formula (Hill Notation) C9H11NO

  • Molecular Weight 149.19

  •  Beilstein Registry Number 4132845

  •  MDL number MFCD00003381

  •  PubChem Substance ID 329760541



Related Categories Aldehydes, Biochemical Reagents for Identification, Building Blocks, C9, Carbonyl Compounds,
grade   for microbiology
shelf life   limited shelf life, expiry date on the label
composition   4-(dimethlyamino)benzaldehyde, 50 g/L
  hydrochloric acid, 240 g/L
  isoamylic alcohol, 710 g/L
Featured Industry   Food and Beverages
storage temp.   2-8°C



Kovac′s reagent may be used for spot test for determination of indole by saturating filter paper with the reagent. It may also be used for initial spot test for determination of indole followed by quantification by spectrophotometric assay and HPLC-UV/Vis-MS/MS methods.

General description

Kovac′s reagent is prepared by mixing p-dimethylaminobenzaldehyde, isoamyl alcohol and concentrated hydrochloric acid. For identification of an organism, the formation of Indole from a tryptophan substrate is a useful diagnostic tool. Indole production is a crucial test in identification of Escherichia coli. After incubation, adding the reagent will help in determination if Indole that has been produced. The reagent turns red when reacts with Indole.

Safety & Documentation

Safety Information

Signal word 
Hazard statements 
UN 2920 3(8) / PGII
WGK Germany 
Flash Point(F) 
109.4 °F
Flash Point(C) 
43 °C

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60983 Kovac’s reagent for indoles, for microbiology

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