• USA Home
  • 67996 - Tris Acetate-EDTA buffer Concentrate

67996 Sigma-Aldrich

Tris Acetate-EDTA buffer Concentrate

for Northern and Southern blotting, solution



Related Categories Buffers, Molecular Biology, Protein Electrophoresis, Proteomics, SDS-PAGE More...
grade   for molecular biology
form   solution
quality   50x Concentrate
impurities   DNases, none detected
  RNases, none detected
  phosphatases, none detected
  proteases, none detected
pH   8.3±0.2 (25 °C)
density   1.09 g/mL at 20 °C
storage temp.   room temp



The TAE 50x liquid concentrate contains 2M TRIS acetate and 0.05M EDTA in water.


Tris Acetate-EDTA buffer concentrate is suitable for use as running buffer for nucleic acid electrophoresis, which may be part of Northern and Southern blot hybridization procedures. It is especially useful for double stranded DNA separations.

General description

TBE (Tris/Acetate/EDTA) Buffer is commonly used in nucleic acid electrophoresis. This solution has a lower buffering capacity than TBE buffer, but double stranded DNA runs faster with TAE buffer. This 50x concentrate can be easily diluted with molecular biology grade water to 1x before use.

Price and Availability

Celebrate Pi Day!
Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 


Certificate of Analysis

Protocols & Articles


Introduction to Nucleic Acid Electrophoresis

Introduction Agarose gel electrophoresis for DNA Agarose gel electrophoresis for RNA Polyacrylamide gel electrophoresis for DNA Reference
Keywords: AGE, Buffers, Degradations, Electrophoresis, Environmental, Gel electrophoresis, PAGE, Purification, Separation, Size-exclusion chromatography

Introduction to Southern and Northern Blotting

The transfer of macromolecules such as nucleic acids and proteins to solid-phase membranous support is known as blotting. Fragments of DNA and RNA molecules separated by gel electrophoresis are trans...
Keywords: AGE, Buffers, Digestions, Electrophoresis, Filtration, Gel electrophoresis, Nucleic acid hybridization, Precipitation, Purification, Scintillation, Separation, Sterilizations

Related Products

related product

Product #


Add to Cart

B6429 Casein Blocking Buffer 10x, for Northern and Southern blotting, solution
D0428 1 kb DNA Ladder, for DNA electrophoresis
H7033 PerfectHyb Plus Hybridization Buffer, for Northern and Southern blotting, solution
T9285 Tris-EDTA buffer solution, 100X concentrate for Northern and Southern blotting
S6639 SSC Buffer 20× Concentrate, for Northern and Southern blotting, solution
B6185 Bionic Buffer, 10X Concentrate, for Northern and Southern blotting, solution
GERPN3005 ECL Direct Nucleic Acid, GE Healthcare, RPN3005, pack of 1 ea
D9156 Deoxyribonucleic acid, single stranded from salmon testes, For hybridization

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?