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Fast Green FCF

analytical standard

Synonym: Food green 3

  • CAS Number 2353-45-9

  • Linear Formula C37H34N2O10S3Na2

  • Molecular Weight 808.85

  •  Colour Index Number 42053

  •  Beilstein/REAXYS Number 5718212

  •  EC Number 219-091-5

  •  MDL number MFCD00013053

  •  PubChem Substance ID 329761116

  •  E Number E143

  •  NACRES NA.24

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Properties

Related Categories Additional Standards, All Alphabetically Sorted, All alphabetical sorted, Analytical Standards, Analytical/Chromatography,
Quality Level   100
grade   analytical standard
assay   ≥98.0% (HPLC)
form   neat
application(s)   HPLC: suitable
  gas chromatography (GC): suitable
mp   290 °C (dec.) (lit.)
Featured Industry   Cleaning Products
Cosmetics
Food and Beverages
Personal Care
format   neat
SMILES string   [Na+].[Na+].CCN(Cc1cccc(c1)S([O-])(=O)=O)c2ccc(cc2)C(=C3/C=CC(\C=C3)=[N+](\CC)Cc4cccc(c4)S([O-])(=O)=O)\c5ccc(O)cc5S([O-])(=O)=O
InChI   1S/C37H36N2O10S3.2Na/c1-3-38(24-26-7-5-9-33(21-26)50(41,42)43)30-15-11-28(12-16-30)37(35-20-19-32(40)23-36(35)52(47,48)49)29-13-17-31(18-14-29)39(4-2)25-27-8-6-10-34(22-27)51(44,45)46;;/h5-23H,3-4,24-25H2,1-2H3,(H3,41,42,43,44,45,46,47,48,49);;/q;2*+1/p-2
InChI key   RZSYLLSAWYUBPE-UHFFFAOYSA-L

Description

General description

Fast Green FCF is a synthetic organic dye, which belongs to the triphenylmethane group. It can be found in a wide range of applications in food and beverage industry. It can also be used in scientific research for the staining of proteins and identification of extracellular recording or injection sites in electrophysiological experiments.

Application

Refer to the product′s Certificate of Analysis for more information on a suitable instrument technique. Contact Technical Service for further support.

Fast Green FCF dye is used for protein staining in IEF, native PAGE and SDS-PAGE. Fast Green staining is linear over a wider range of protein concentrations than Brilliant Blue R. After electrophoresis, fixing the proteins in the gel is recommended for maximum sensitivity. The gel can then be stained for 2 hr with 0.1% Fast Green FCF in either 30% (v/v) ethanol, 10% (v/v) acetic acid or in 7% acetic acid. The gel can be destained with either 30% (v/v) ethanol, 10% (v/v) acetic acid, or 7% acetic acid. The stained gel can be scanned at 625 nm. The detection sensitivity is approx. 30% that with Brilliant Blue R.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Safety & Documentation

Safety Information

Symbol 
GHS08  GHS08
Signal word 
Warning
Hazard statements 
Precautionary statements 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 2
RTECS 
BQ4425000
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

HPLC Analysis of Synthetic Food Dyes on Ascentis® Express C18

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Synthetic Food Dyes on Ascentis® Express C18
Keywords: Chromatography, High performance liquid chromatography

Peer-Reviewed Papers
15

References

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