70184 Millipore

Antibiotic Broth

for microbiology, NutriSelect Plus

Synonym: Antibiotic Medium No. 3, Assay Broth, Penassay Broth, Grove and Randall Antibiotic Broth

  •  NACRES NA.74



Related Categories A - M, All Liquid Media/Broths, Clinical Media, Escherichia coli & Coliforms, Klebsiella,
Quality Level   100
grade   for microbiology
form   powder
shelf life   limited shelf life, expiry date on the label
composition   dextrose, 1.0 g/L
  dipotassium hydrogen phosphate, 3.68 g/L
  meat extract, 1.5 g/L
  peptone (from meat), 5.0 g/L
  potassium dihydrogen phosphate, 1.32 g/L
  sodium chloride, 3.5 g/L
  yeast extract, 1.5 g/L
mfr. no.   NutriSelect Plus
application(s)   microbiological culture: suitable
final pH   7.0±0.2 (25 °C)
Featured Industry   Clinical Testing
Food and Beverages
Life Science and Biopharma
suitability   nonselective for Escherichia coli
  nonselective for Klebsiella spp.
  nonselective for Staphylococcus spp.
  nonselective for Streptococcus spp.
  nonselective for coliforms



Used in antibiotic assay work (USP, FDA, Ph Eur) according to Grove & Randall; used in the turbidimetric assay of e.g. penicillin, bacitracin and kanamycin with S. aureus and for the cylinder assay of e.g. erythromycin and chloramphenicol with M. inteus.

Preparation Note

Dissolve 17.5 g in 1 litre of warm (60 C) distilled water and sterilize by autoclaving at 121°C for 15 minutes.

Legal Information

NutriSelect is a trademark of Merck KGaA, Darmstadt, Germany

Safety & Documentation

Safety Information

Personal Protective Equipment 
NONH for all modes of transport
WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable


Certificate of Analysis (COA)

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Protocols & Articles


Microbiology Introduction

An initial aim of all microbiologists is the reproducible growth of their microbial cultures, no matter whether the microorganisms are of natural origin or have been genetically engineered by man. Re...
Keywords: Absorption, Aerobic, Anaerobic, Antibiotics, Antimicrobials, Buffers, Culture media, Diffusion, Environmental, Filtration, Genetic, Growth factors, Metabolism, Phase transitions, Precipitation, Reductions, Respiration, Sample preparations, Vitamins

Microbiology Theory: Media Preparation

Microorganisms need nutrients, a source of energy and certain environmental conditions in order to grow and reproduce. In the natural environment, microbes have adapted to the habitats most suitable ...
Keywords: Culture media, Digestions, Environmental, Filtration, Growth factors, Indicators, Microbiology, Phase transitions, Precipitation, Vitamins

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