70186 Millipore

EMB Agar

for microbiology

Synonym: Eosin Methylene Blue Agar



Related Categories A - M, All Dairy Media (alphabetical), All Food Control Media (alphabetical), All Solid Media/Agars, Candida,
grade   for microbiology
shelf life   limited shelf life, expiry date on the label
composition   agar, 15.0 g/L
  dipotassium hydrogen phosphate, 2.0 g/L
  eosin Y, 0.4 g/L
  lactose, 10.0 g/L
  methylene blue, 0.065 g/L
  peptone, 10.0 g/L
final pH   7.1±0.2 (25 °C)
Featured Industry   Clinical Testing
Cosmetics, Personal Care, and Cleaning Products
Food and Beverages
suitability   slightly selective and differential for Candida
  slightly selective and differential for Citrobacter
  slightly selective and differential for Enterobacter
  slightly selective and differential for Enterobacteriaceae
  slightly selective and differential for Enterococcus
  slightly selective and differential for Escherichia coli and Coliforms
  slightly selective and differential for Klebsiella
  slightly selective and differential for Proteus
  slightly selective and differential for Salmonella
  slightly selective and differential for Staphylococcus



A versatile solid medium, originally developed by Levine, for the differentiation of Eschericha coli and Aerobacter aerogenes, for the rapid identification of Candida albicans and for the identification of coagulase-positive Staphylococci.

Other Notes

Recommended by the American Public Health Association (APHA) and the United States Pharmacopeia (USP); Compendium of Methods for the Microbiological Examination of Foods; Enumeration of E. coli from lake water.

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 


Certificate of Analysis

Protocols & Articles


Microbiology Introduction

An initial aim of all microbiologists is the reproducible growth of their microbial cultures, no matter whether the microorganisms are of natural origin or have been genetically engineered by man. Re...
Keywords: Absorption, Aerobic, Anaerobic, Antibiotics, Antimicrobials, Buffers, Culture media, Diffusion, Environmental, Filtration, Genetic, Growth factors, Metabolism, Phase transitions, Precipitation, Reductions, Respiration, Sample preparations, Vitamins

Microbiology Theory: Media Preparation

Microorganisms need nutrients, a source of energy and certain environmental conditions in order to grow and reproduce. In the natural environment, microbes have adapted to the habitats most suitable ...
Keywords: Culture media, Digestions, Environmental, Filtration, Growth factors, Indicators, Microbiology, Phase transitions, Precipitation, Vitamins


Yeast is one of the most important microorganisms known and utilised by mankind. Ancient Middle Eastern civilisations used the organism to bake bread and to produce mead, beer and wine.
Jvo Siegrist
Analytix 2009, 5, 7.
Keywords: Antibiotics, Enzyme-linked immunosorbent assay, Fermentation, Molecular biology, Polymerase chain reaction, Reproduction, Vitamins

Peer-Reviewed Papers


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