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70189 Millipore

Violet Red Bile Glucose Agar

for microbiology, NutriSelect Plus

Synonym: VRBG-Agar

  •  NACRES NA.85

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Properties

Related Categories All Dairy Media (alphabetical), All Food Control Media (alphabetical), All Solid Media/Agars, Citrobacter, Enterobacteriaceae,
Quality Level   100
grade   for microbiology
form   powder
shelf life   limited shelf life, expiry date on the label
composition   agar, 13.0 g/L
  bile salts, 1.5 g/L
  crystal violet, 0.002 g/L
  dextrose, 10.0 g/L
  neutral red, 0.03 g/L
  peptone (from gelatin), 7.0 g/L
  sodium chloride, 5.0 g/L
  yeast extract, 3.0 g/L
mfr. no.   NutriSelect Plus
application(s)   bioburden testing: suitable
  microbiological culture: suitable
  water monitoring: suitable
final pH   7.2±0.2 (25 °C)
Featured Industry   Agriculture
Environmental
Food and Beverages
suitability   selective and differential for Citrobacter spp.
  selective and differential for Enterococcus spp.
  selective and differential for Escherichia coli
  selective and differential for Klebsiella spp.
  selective and differential for Proteus spp.
  selective and differential for Salmonella spp.
  selective and differential for Shigella spp.
  selective and differential for coliforms
  selective and differential for enterobacteriaceae

Description

Application

A selective medium for the isolation and enumeration of enterobacteria.

Preparation Note

Suspend 39.5g in 1 litre of distilled water and let soak. Bring to the boil to fully dissolve the powder. Sterilize by boiling for 1 minute with frequent agitation. DO NOT AUTOCLAVE. Prolonged heating in thermostatic bath could cause slight precipitates.

Other Notes

Enumeration of Enterobacteriaceae in milk

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Differentiation and Identification Media for Salmonella

Salmonella contamination is the second leading cause of food-borne illness worldwide. Controlling outbreaks of Salmonella is an important task for food regulators, restaurants and the food industry i...
Jvo Siegrist
Microbiology Focus Edition 1.3
Keywords: Biochemistry, Fermentation, Food & Beverage, Indicators, Microbiology

Enterobacteriaceae in Food

From our library of Articles, Sigma-Aldrich presents Enterobacteriaceae in Food

Microbiology Introduction

An initial aim of all microbiologists is the reproducible growth of their microbial cultures, no matter whether the microorganisms are of natural origin or have been genetically engineered by man. Re...
Keywords: Absorption, Aerobic, Anaerobic, Antibiotics, Antimicrobials, Buffers, Culture media, Diffusion, Environmental, Filtration, Genetic, Growth factors, Metabolism, Phase transitions, Precipitation, Reductions, Respiration, Sample preparations, Vitamins

Microbiology Theory: Media Preparation

Microorganisms need nutrients, a source of energy and certain environmental conditions in order to grow and reproduce. In the natural environment, microbes have adapted to the habitats most suitable ...
Keywords: Culture media, Digestions, Environmental, Filtration, Growth factors, Indicators, Microbiology, Phase transitions, Precipitation, Vitamins

Peer-Reviewed Papers
15

References

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