70191 Millipore

Mueller Hinton Agar

for microbiology

  •  NACRES NA.74



Related Categories A - M, All Solid Media/Agars, Bacteria (General Media), Campylobacter, Clinical Media,
Quality Level   100
grade   for microbiology
form   powder
shelf life   limited shelf life, expiry date on the label
composition   agar, 17.0 g/L
  beef infusion solids, 2.0 g/L
  casein hydrolysate, 17.5 g/L
  starch, 1.5 g/L
application(s)   microbiological culture: suitable
final pH   7.3±0.2 (25 °C)
Featured Industry   Clinical Testing
Food and Beverages
suitability   nonselective for Neisseria spp.
  nonselective for bacteria (General Media)



Mueller Hinton Agar is a solid medium originally designed for the isolation of pathogenic Neisseria species, now widely used for antibiotic susceptibility testing (including sulfonamides). Mueller Hinton Agar has been used:
• in culturing Escherichia coli ATCC® 25922 strain for disc diffusion studies
• in disk diffusion assay of fusidic acid against Staphylococcus aureus, Bacillus cereus, Pseudomonas aeruginosa and Escherichia coli
• in agar diffusion assay of drug loaded nano-hydroxyapatite formulations against Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli

Other Notes

Enhancement of cefotoxamine and other cephalosporins against Enterococcus faecalis by blood supplemented Mueller-Hinton agar

Legal Information

ATCC is a registered trademark of American Type Culture Collection

Safety & Documentation

Safety Information

Personal Protective Equipment 
NONH for all modes of transport
WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable


Certificate of Analysis (COA)

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Protocols & Articles


Campylobacter Detection: Rapid Testing Solutions for the Detection of Campylobacter

Campylobacter spp. are regarded as the leading cause of bacterial gastroenteritis in humans worldwide. Infections are usually caused by consuming cross-contaminated or insufficiently processed food (...
Keywords: Adhesion, Aerobic, Antibiotics, Clinical, Culture media, Gastrointestinal, Gene expression, Homogenization, Microbiology, Polymerase chain reaction

Microbiology Introduction

An initial aim of all microbiologists is the reproducible growth of their microbial cultures, no matter whether the microorganisms are of natural origin or have been genetically engineered by man. Re...
Keywords: Absorption, Aerobic, Anaerobic, Antibiotics, Antimicrobials, Buffers, Culture media, Diffusion, Environmental, Filtration, Genetic, Growth factors, Metabolism, Phase transitions, Precipitation, Reductions, Respiration, Sample preparations, Vitamins

Microbiology Theory: Media Preparation

Microorganisms need nutrients, a source of energy and certain environmental conditions in order to grow and reproduce. In the natural environment, microbes have adapted to the habitats most suitable ...
Keywords: Culture media, Digestions, Environmental, Filtration, Growth factors, Indicators, Microbiology, Phase transitions, Precipitation, Vitamins

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