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70439 Millipore

Oxidase Test

for microbiology

  •  eCl@ss 32161000

  •  NACRES NA.85

Purchase

Properties

Related Categories Discs, Strips and Cards, General Identification & Differentiation, Identification Tests & Reagents, Microbiology
Quality Level   100
grade   for microbiology
shelf life   limited shelf life, expiry date on the label
application(s)   microbiology: suitable (microbial identification)
Featured Industry   Agriculture
Clinical Testing
Environmental
Food and Beverages
Pharmaceutical
suitability   bacteria

Description

Application

Oxidase Test is an important differential procedure which should be performed on all gram-negative bacteria that are to be identified.

Packaging

Discs contain N,N-dimethyl-p-phenylenediamine oxalate and α-naphthol.

Principle

Oxidase Test is based on the principle that certain bacteria produce indophenol blue from the oxidation of dimethyl-p-phenylenediamine and α-naphthol. In presence of the enzyme cytochrome oxidase (gram-negative bacteria), the N,N-dimethyl-p-phenylenediamine oxalate and α-naphthol react to indophenol blue.

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

Please Enter a Lot Number
Protocols & Articles

Articles

Differentiation of Escherichia coli from coliforms

In August 2008, the discovery of E. coli-contaminated beef in the United States prompted a nationwide recall of beef. The source turned out to be one supplier that had a history of contamination of i...
Jvo Siegrist
AnalytiX Volume 8 Article 5
Keywords: Decarboxylations, Fermentation, Genetic, Indicators, Microbiology, Reductions

Enterobacteriaceae in Food

From our library of Articles, Sigma-Aldrich presents Enterobacteriaceae in Food

Identification of Microorganisms Based on Color

For microbiologists the most fundamental stain was developed in 1884 by the Danish bacteriologist Hans Christian Gram. The Gram Stain allows the determination of morphology, dividing bacteria into tw...
Jvo Siegrist
Microbiology Focus Edition 1.4, Product Manager Microbiology.... ivo.siegrist@sial.com
Keywords: Antibiotics, Enzyme activity, Fermentation, Gram stain, Help, Indicators, Reductions

Microbiology Introduction

An initial aim of all microbiologists is the reproducible growth of their microbial cultures, no matter whether the microorganisms are of natural origin or have been genetically engineered by man. Re...
Keywords: Absorption, Aerobic, Anaerobic, Antibiotics, Antimicrobials, Buffers, Culture media, Diffusion, Environmental, Filtration, Genetic, Growth factors, Metabolism, Phase transitions, Precipitation, Reductions, Respiration, Sample preparations, Vitamins

On the Trail of Campylobacter

The Campylobacter is one of the leading causes of human gastroenteritis. Common Campylobacter species C. jejuni, C. coli, and C. lari are responsible for most cases of campylobacteriosis1,2. However,...
Jvo Siegrist
AnalytiX Volume 8 Issue 1
Keywords: Antibiotics, Cancer, Citric Acid Cycle, Gram stain, Metabolism, Microbiology, Scanning electron microscopy

Pseudomonas Media and Tests

Pseudomonas are motile (one or more polar flagella), rod shaped and aerobe Gram-negative bacteria. They are found almost everywhere, in soil, water, plants and animals. In most cases it is not pathog...
Jvo Siegrist, Product Manager Microbiology, ivo.siegrist@sial.com
Analytix Volume 2007 Article 5
Keywords: Anaerobic, Antifungals, Biochemistry, Cosmetics, Diagnostic, Environmental, Fermentation, Glycolysis, Metabolism, Metabolites, Microbiology, Pentose phosphate pathway, Pharmaceutical, Respiratory

Reliable Testing Solutions & Media for Detection of Staphylococcus Aureus: a Spreading Bacteria

Staphylococcus aureus is frequently a part of our skin flora but is also a cause of a broad range of illnesses. Current studies report a remarkable increase of Methicillin Resistant Staphylococcus au...
Jvo Siegrist
AnalytiX Volume 10 Article 2
Keywords: Aerobic, Antibiotics, Clinical, Environmental, Fermentation, Food & Beverage, Microbiology, Respiration

Vibrio Detection

Vibrios are motile, curved or comma-shaped bacilli and have a single polar flagella with sheet proteins. They are often found in open water, freshwater and saltwater. Vibrios are facultative aerobe a...
Jvo Siegrist
Microbiology Focus Edition 1.3
Keywords: Dehydration reaction, Diagnostic, Fermentation, Indicators, Metabolism, Microbiology

Yersinia Diagnostic

Yersinia are rod-shaped, Gram-negative facultative anaerobic bacteria. They exhibit fermentative metabolism and are oxidasenegative, mannitol-positive. A psychrophilic organism, Yersinia survives and...
Ivo Siegrist
Analytix Volume 7 Article 5
Keywords: Anaerobic, Clinical, Diagnostic, Metabolism, Microbiology

Related Content

Biomonitoring

We offer a complete range of high quality microbial testing solutions for the pharmaceutical, cosmetics, municipal water, beverages and food industries. For that one invaluable result: safe products.
Keywords: Cosmetics, Industries, Pharmaceutical

Peer-Reviewed Papers
15

References

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