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70722 Millipore

E. coli ChromoSelect Agar B

suitable for microbiology, NutriSelect® Plus

Synonym: E. coli Agar B ChromoSelect

  •  NACRES NA.85

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Properties

Related Categories A - M, All Food Control Media (alphabetical), All Solid Media/Agars, Chromogenic Media, Escherichia coli & Coliforms,
Quality Level   100
form   powder
shelf life   limited shelf life, expiry date on the label
composition   agar, 15 g/L
  bile salt mixture, 1.5 g/L
  casein enzymic hydrolysate, 20 g/L
  X-glucuronide, 0.075 g/L
mfr. no.   NutriSelect® Plus
application(s)   bioburden testing: suitable
  microbiology: suitable (microbial identification)
  water monitoring: suitable
final pH   7.2±0.2 (25 °C)
Featured Industry   Agriculture
Environmental
Food and Beverages
suitability   selective and differential for Escherichia coli
  selective and differential for Klebsiella spp.
  selective and differential for coliforms

Description

Application

E. coli ChromoSelect Agar B is recommended for the detection and enumeration of Escherichia coli in foods without further confirmation on membrane filter or by indole reagent. Most of the Escherichia coli strains can be differentiated from other coliforms by the presence of enzyme glucuronidase which is highly specific for Escherichia coli. Escherichia coli cells absorb X-glucuronide and the intracellular glucuronidase splits the bond between the chromophore and the glucuronide. The released chromophore gives the blue coloration of the colonies.

Preparation Note

Suspend 36.6 g per litre in distilled water. Sterilize by autoclaving at 121°C for 15 minutes. Cool to 50°C and pour into sterile petri plates.

Legal Information

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Microbiology Introduction

An initial aim of all microbiologists is the reproducible growth of their microbial cultures, no matter whether the microorganisms are of natural origin or have been genetically engineered by man. Re...
Keywords: Absorption, Aerobic, Anaerobic, Antibiotics, Antimicrobials, Buffers, Culture media, Diffusion, Environmental, Filtration, Genetic, Growth factors, Metabolism, Phase transitions, Precipitation, Reductions, Respiration, Sample preparations, Vitamins

Microbiology Theory: Media Preparation

Microorganisms need nutrients, a source of energy and certain environmental conditions in order to grow and reproduce. In the natural environment, microbes have adapted to the habitats most suitable ...
Keywords: Culture media, Digestions, Environmental, Filtration, Growth factors, Indicators, Microbiology, Phase transitions, Precipitation, Vitamins

Overview of Chromogenic Media

The use of traditional versus improved media formulation containing chromogenic substrate is currently an important topic in the field of microbiology. The focus behind such developments was to produ...
Jvo SiegristProduct Manager
AnalytiX Issue 3
Keywords: Culture media, Diffusion, Electron capture detector, Fermentation, Filtration, Metabolites, Microbiology

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