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71015BC Sigma-Aldrich

GDS MPX Top 7 STEC

BioControl, Molecular based PCR test for the detection of E. coli O157:H7 and the "Top Six" non-O157 Shiga Toxigenic E. coli (STEC) in food

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Properties

shelf life   6‑12 mo.
mfr. no.   BioControl Systems, 71015-100
  BioControl Systems, 71015-576
storage temp.   2-8°C

Description

Components

• MPX Top STEC Amplification Tubes
• Top 7 STEC Concentration ReagentResuspension Buffer TqTop STEC Wash Solution

Testing Method

PCR, Immunomagnetic Separation (IMS)

Compatibility

BioControl GDS

Application

AOAC Performance Tested Method 071301

Features and Benefits

• Following sample preparation with IMS against the Top 7 O groups, samples are analyzed with a single multiplex assay for the presence of (eae), stx1, and stx2
• Assurance GDS MPX Top 7 STEC assay includes a separate target and differentiated result for E. coli O157:H7
• Single-step 10-18 h enrichment in mEHEC®, enrichment media common to all BioControl methods for E.coli O157:H7, Shiga Toxin genes, and Top STEC

General description

GDS MPX Top 7 STEC is an automated nucleic acid amplification system for the detection of E. coli O157:H7 and the “Top Six” non-O157 Shiga Toxigenic E. coli (STEC) in raw ground beef, raw beef trim, raw spinach, and raw mixed greens (baby lettuce and other young leafy green vegetables). The "Top Six" non-O157 STEC are defined as E. coli belonging to serogroups O103, O111, O121, O145, O26 or O45 that possess both the eae gene and at least one of the Shiga toxin genes stx1 or stx2.

Assurance GDS MPX Top 7 STEC utilizes a proprietary IMS-based sample preparation procedure to capture organisms belonging to 7 specific Top STEC O-serogroups (O103, O111, O121, O145, O26, O45 and O157) prior to genetic analysis for the associated pathogenicity genes.

Legal Information

mEHEC is a registered trademark of BioControl Systems, Inc.

Safety & Documentation

Safety Information

RIDADR 
UN 3316 9

Documents

Certificate of Analysis

Protocols & Articles
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