• USA Home
  • 71017BC - GDS Top 7 STEC (eae) Tq

71017BC Millipore

GDS Top 7 STEC (eae) Tq

BioControl, Molecular based PCR test for the detection of the eae gene in "Top Seven" Shiga Toxigenic E. coli (STEC) in food



Agency/Method   AOAC® (PTM)
shelf life   6‑12 mo.
packaging   pkg of 100 tests
mfr. no.   Assurance GDS®
  BioControl Systems, 71017-100
application(s)   PCR: suitable
Featured Industry   Food and Beverages
storage temp.   2-8°C
suitability   enterovirulent E. coli (EEC) (shiga toxigenic)


General description

GDS Top 7 STEC (eae) Tq is an automated nucleic acid amplification system for the detection of the eae gene in the following select O-serogroups of E. coli: O103, O111, O121, O145, O26, O45 and O157. Assurance GDS utilizes a proprietary IMS-based sample preparation procedure to isolate organisms belonging to these specific 7 O-serogroups prior to genetic analysis for the presence of the eae gene in a variety of foods, including raw ground beef, raw beef trim, raw spinach, and raw mixed greens.

Assurance GDS Top 7 STEC (eae) Tq is designed for use in conjunction with Assurance GDS Shiga Toxin Genes (Top 7) Tq to detect the presence of Shiga Toxigenic E. coli belonging to O-serogroups O103, O111, O121, O145, O26, O45 or O157.


AOAC Performace Tested Method 071303

Features and Benefits

• Sequential approach to detecting Top STEC. Following sample preparation with IMS against the Top 7 O groups, samples are analyzed for the presence of (eae) with the GDS Top 7 STEC (eae) assay. Positive samples are subsequently analyzed for Shiga Toxin genes with the Assurance GDS Shiga Toxin Genes Top 7 assay.
• Top 7 STEC (includes E. coli O157:H7)
• Single-step 10-18 h enrichment in mEHEC, enrichment media common to all BioControl methods for E.coli O157:H7, Shiga Toxin genes, and Top STEC


• Top STEC (eae) Amplification Tubes Tq
• Top 7 STEC Concentration Reagent
• Resuspension Buffer Tq
• Top STEC Wash Solution


BioControl GDS

Testing Method

PCR, Immunomagnetic Separation (IMS)

Legal Information

AOAC is a registered trademark of AOAC International

Safety & Documentation

Safety Information

UN 3316 9


Certificate of Analysis (COA)

Please Enter a Lot Number
Protocols & Articles
Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?