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71017BC Millipore

GDS Top 7 STEC (eae) Tq

BioControl, Molecular based PCR test for the detection of the eae gene in "Top Seven" Shiga Toxigenic E. coli (STEC) in food



shelf life   6‑12 mo.
packaging   pkg of 100 tests
mfr. no.   Assurance GDS®
  BioControl Systems, 71017-100
application(s)   PCR: suitable
Agency/Method   AOAC® (PTM)
Featured Industry   Food and Beverages
storage temp.   2-8°C
suitability   enterovirulent E. coli (EEC) (shiga toxigenic)


General description

GDS Top 7 STEC (eae) Tq is an automated nucleic acid amplification system for the detection of the eae gene in the following select O-serogroups of E. coli: O103, O111, O121, O145, O26, O45 and O157. Assurance GDS utilizes a proprietary IMS-based sample preparation procedure to isolate organisms belonging to these specific 7 O-serogroups prior to genetic analysis for the presence of the eae gene in a variety of foods, including raw ground beef, raw beef trim, raw spinach, and raw mixed greens.

Assurance GDS Top 7 STEC (eae) Tq is designed for use in conjunction with Assurance GDS Shiga Toxin Genes (Top 7) Tq to detect the presence of Shiga Toxigenic E. coli belonging to O-serogroups O103, O111, O121, O145, O26, O45 or O157.


AOAC Performace Tested Method 071303

Features and Benefits

• Sequential approach to detecting Top STEC. Following sample preparation with IMS against the Top 7 O groups, samples are analyzed for the presence of (eae) with the GDS Top 7 STEC (eae) assay. Positive samples are subsequently analyzed for Shiga Toxin genes with the Assurance GDS Shiga Toxin Genes Top 7 assay.
• Top 7 STEC (includes E. coli O157:H7)
• Single-step 10-18 h enrichment in mEHEC, enrichment media common to all BioControl methods for E.coli O157:H7, Shiga Toxin genes, and Top STEC


• Top STEC (eae) Amplification Tubes Tq
• Top 7 STEC Concentration Reagent
• Resuspension Buffer Tq
• Top STEC Wash Solution


BioControl GDS

Testing Method

PCR, Immunomagnetic Separation (IMS)

Legal Information

AOAC is a registered trademark of AOAC International

Safety & Documentation

Safety Information

UN 3316 9


Certificate of Analysis (COA)

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