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71017BC Sigma-Aldrich

GDS Top 7 STEC (eae) Tq

BioControl, Molecular based PCR test for the detection of the eae gene in "Top Seven" Shiga Toxigenic E. coli (STEC) in food

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Properties

shelf life   6‑12 mo.
mfr. no.   BioControl Systems, 71017-100
storage temp.   2-8°C

Description

Components

• Top STEC (eae) Amplification Tubes Tq
• Top 7 STEC Concentration Reagent
• Resuspension Buffer Tq
• Top STEC Wash Solution

Testing Method

PCR, Immunomagnetic Separation (IMS)

Compatibility

BioControl GDS

Application

AOAC Performace Tested Method 071303

Features and Benefits

• Sequential approach to detecting Top STEC. Following sample preparation with IMS against the Top 7 O groups, samples are analyzed for the presence of (eae) with the GDS Top 7 STEC (eae) assay. Positive samples are subsequently analyzed for Shiga Toxin genes with the Assurance GDS Shiga Toxin Genes Top 7 assay.
• Top 7 STEC (includes E. coli O157:H7)
• Single-step 10-18 h enrichment in mEHEC, enrichment media common to all BioControl methods for E.coli O157:H7, Shiga Toxin genes, and Top STEC

General description

GDS Top 7 STEC (eae) Tq is an automated nucleic acid amplification system for the detection of the eae gene in the following select O-serogroups of E. coli: O103, O111, O121, O145, O26, O45 and O157. Assurance GDS utilizes a proprietary IMS-based sample preparation procedure to isolate organisms belonging to these specific 7 O-serogroups prior to genetic analysis for the presence of the eae gene in a variety of foods, including raw ground beef, raw beef trim, raw spinach, and raw mixed greens.

Assurance GDS Top 7 STEC (eae) Tq is designed for use in conjunction with Assurance GDS Shiga Toxin Genes (Top 7) Tq to detect the presence of Shiga Toxigenic E. coli belonging to O-serogroups O103, O111, O121, O145, O26, O45 or O157.

Safety & Documentation

Safety Information

RIDADR 
UN 3316 9

Documents

Certificate of Analysis

Protocols & Articles
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