80457 Supelco

Trifluoroacetic acid

eluent additive for LC-MS, LiChropur, ≥99.0% (GC)

Synonym: TFA

  • CAS Number 76-05-1

  • Linear Formula CF3COOH

  • Molecular Weight 114.02

  •  Beilstein/REAXYS Number 742035

  •  EC Number 200-929-3

  •  MDL number MFCD00004169



Related Categories Analytical Reagents, Analytical/Chromatography, Chromatography Reagents & Solvents, LC-MS Additives & Reagents, LC-MS Mobile Phase Additives,
Quality Level   100
grade   eluent additive for LC-MS
vapor density   3.9 (vs air)
vapor pressure   97.5 mmHg ( 20 °C)
assay   ≥99.0% (GC)
application(s)   HPLC: suitable
  LC/MS: suitable
impurities   ≤0.05% water
  ≤50 mg/kg total sulfur (as SO4)
refractive index   n20/D 1.3 (lit.)
bp   72.4 °C (lit.)
mp   −15.4 °C (lit.)
density   1.489 g/mL at 20 °C (lit.)
anion traces   chloride (Cl-): ≤50 mg/kg
  sulfite (SO32-): ≤10 mg/kg
cation traces   Ag: ≤0.05 mg/kg
  Al: ≤0.1 mg/kg
  Ba: ≤0.1 mg/kg
  Bi: ≤0.5 mg/kg
  Ca: ≤0.5 mg/kg
  Cd: ≤0.1 mg/kg
  Co: ≤0.05 mg/kg
  Cr: ≤0.1 mg/kg
  Cu: ≤0.05 mg/kg
  Fe: ≤1.0 mg/kg
  K: ≤1.0 mg/kg
  Li: ≤0.1 mg/kg
  Mg: ≤1.0 mg/kg
  Mn: ≤0.1 mg/kg
  Mo: ≤0.05 mg/kg
  NH4+: ≤10 mg/kg
  Na: ≤1.0 mg/kg
  Ni: ≤0.1 mg/kg
  Pb: ≤0.05 mg/kg
  Sr: ≤0.05 mg/kg
  Tl: ≤0.1 mg/kg
  Zn: ≤0.1 mg/kg
UV absorption   λ: 260 nm Amax: ≤0.9
  λ: 270 nm Amax: ≤0.1
  λ: 280 nm Amax: ≤0.05
  λ: 290 nm Amax: ≤0.04
  λ: 300 nm Amax: ≤0.03
  λ: 320 nm Amax: ≤0.025
SMILES string   OC(C(F)(F)F)=O
suitability   passes test for LC-MS
InChI   1S/C2HF3O2/c3-2(4,5)1(6)7/h(H,6,7)


General description

Trifluoroacetic acid is commonly used as a mobile phase additive in liquid chromatography-mass spectrometry (LC-MS).
• Extensive QC testing ensures the highest specifications.        
• Optimized to improve ionization and resolution.        
• Extremely low levels of inorganic and organic impurities.       
• Manufactured specifically for accurate and fast LC-MS.


Trifluoroacetic acid may be used as a mobile phase additive for the analysis of basic compounds in biological samples using hydrophilic interaction liquid chromatography-electrospray ionization coupled to tandem mass spectrometry (HILIC–ESI/MS/MS) and reversed-phase liquid chromatography coupled to tandem mass spectrometry (RP-LC-MS/MS).


Clear borosilicate glass, high hydrolytic resistance (Type I)

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Safety & Documentation

Safety Information

Signal word 
Hazard statements 
UN 2699 8 / PGI
WGK Germany 
Flash Point(F) 
Pensky-Martens closed cup - Not applicable
Flash Point(C) 
Pensky-Martens closed cup - Not applicable
Protocols & Articles


Determination of Lactose in dairy products using TLC-MS

Today food intolerances play a big role in our society. For example a lot of people are lactose intolerant, usually some more in Asian countries than in Europe or North America. So far, analysis of l...
Keywords: Centrifugation, Chromatography, Gene expression, High performance liquid chromatography, Industries, Liquid chromatography mass spectrometry, Mass spectrometry, PAGE, Sample preparations, Spectroscopy, Thin layer chromatography

LC/MS Analysis of Peptides on Discovery® BIO Wide Pore C18, Effect of TFA Concentration on MS Signal

From our library of Articles, Sigma-Aldrich presents LC/MS Analysis of Peptides on Discovery® BIO Wide Pore C18, Effect of TFA Concentration on MS Signal
Keywords: Chromatography, High performance liquid chromatography

Peer-Reviewed Papers


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