85463 Millipore

Simmons Citrate Agar

for microbiology

Synonym: Citrate Utilization Test

  •  NACRES NA.74



Related Categories All Food Control Media (alphabetical), All Solid Media/Agars, Biochemical Identification Media by Test, Biochemical Identification Media, A to Z, Carbon Utilization Test,
Quality Level   100
grade   for microbiology
form   powder
shelf life   limited shelf life, expiry date on the label
composition   agar, 15 g/L
  ammonium dihydrogen phosphate, 0.2 g/L
  bromothymol blue, 0.08 g/L
  disodium ammonium phosphate, 0.8 g/L
  magnesium sulfate heptahydrate, 0.2 g/L
  sodium chloride, 5 g/L
  trisodium citrate, 2 g/L
final pH   ~7.0
Featured Industry   Agriculture
Clinical Testing
Food and Beverages
suitability   nonselective and differential for Escherichia coli
  nonselective and differential for Salmonella spp.
  nonselective and differential for coliforms
  nonselective and differential for molds (General Media)
  nonselective and differential for yeasts (General Media)


General description

Simmons Citrate Agar uses citric acid as the carbon source. It is used to differentiate Salmonella groups from each other. It does not allow the growth of Shigella or coliforms.


Simmons Citrate Agar can be used for differentiating between fecal coli and members of the aerogenes group based on citrate utilization. It can also be used for the identification of certain fungi and fungi imperfecti. The agar has been used to perform a citrate test.

Other Notes

Selective recovery of Klebsiella pneumoniae and Klebsiella oxytoca from human feces

Safety & Documentation

Safety Information

Personal Protective Equipment 
NONH for all modes of transport
WGK Germany 


Certificate of Analysis (COA)

Please Enter a Lot Number
Protocols & Articles


Differentiation and Identification Media for Salmonella

Salmonella contamination is the second leading cause of food-borne illness worldwide. Controlling outbreaks of Salmonella is an important task for food regulators, restaurants and the food industry i...
Jvo Siegrist
Microbiology Focus Edition 1.3
Keywords: Biochemistry, Fermentation, Food & Beverage, Indicators, Microbiology

Differentiation of Escherichia coli from coliforms

In August 2008, the discovery of E. coli-contaminated beef in the United States prompted a nationwide recall of beef. The source turned out to be one supplier that had a history of contamination of i...
Jvo Siegrist
AnalytiX Volume 8 Article 5
Keywords: Decarboxylations, Fermentation, Genetic, Indicators, Microbiology, Reductions

Microbiology Introduction

An initial aim of all microbiologists is the reproducible growth of their microbial cultures, no matter whether the microorganisms are of natural origin or have been genetically engineered by man. Re...
Keywords: Absorption, Aerobic, Anaerobic, Antibiotics, Antimicrobials, Buffers, Culture media, Diffusion, Environmental, Filtration, Genetic, Growth factors, Metabolism, Phase transitions, Precipitation, Reductions, Respiration, Sample preparations, Vitamins

Microbiology Theory: Media Preparation

Microorganisms need nutrients, a source of energy and certain environmental conditions in order to grow and reproduce. In the natural environment, microbes have adapted to the habitats most suitable ...
Keywords: Culture media, Digestions, Environmental, Filtration, Growth factors, Indicators, Microbiology, Phase transitions, Precipitation, Vitamins

Peer-Reviewed Papers


Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?