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857343 Sigma-Aldrich

Basic Fuchsin

certified by the Biological Stain Commission, Dye content ≥88 %

Synonym: Basic Parafuchsin, Basic Red 9, Magenta O, Parafuchsin hydrochloride, Paramagenta hydrochloride, Pararosaniline chloride, Pararosaniline hydrochloride

  • CAS Number 569-61-9

  • Empirical Formula (Hill Notation) C19H17N3 · HCl

  • Molecular Weight 323.82

  •  Colour Index Number 42500

  •  Beilstein/REAXYS Number 4164603

  •  EC Number 209-321-2

  •  MDL number MFCD00001657

  •  PubChem Substance ID 24888442

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Properties

Related Categories B, Cell Biology, Certified Stains & Dyes, Hematology and Histology, Stains & Dyes, A to Z,
Quality Level   200
grade   certified by the Biological Stain Commission
form   powder
composition   Dye content, ≥88%
application(s)   hematology: suitable
  histology: suitable
pH range   1.0 - 3.1, purple to red
mp   268-270 °C (dec.) (lit.)
λmax   544 nm
ε (extinction coefficient)   ≥11000 at 235-239 nm in 50% ethanol at 0.003 g/L
  ≥17000 at 287-291 nm in 50% ethanol at 0.003 g/L
Featured Industry   Diagnostic Assay Manufacturing
storage temp.   room temp
SMILES string   [Cl-].Nc1ccc(cc1)C(\c2ccc(N)cc2)=C3/C=CC(=[NH2+])C=C3
InChI   1S/C19H17N3.ClH/c20-16-7-1-13(2-8-16)19(14-3-9-17(21)10-4-14)15-5-11-18(22)12-6-15;/h1-12,20H,21-22H2;1H
InChI key   JUQPZRLQQYSMEQ-UHFFFAOYSA-N

Description

Application

Basic fuchsin has been used as a lignin-detecting fluorogenic dye for fluorescence-based imaging of lignin in the basal internodes of three Brachypodium distachyon ecotypes.

Packaging

25, 100 g in glass bottle

Biochem/physiol Actions

Basic fuchsin belongs to the triphenylmethane group. Combining basic fuchsin with sulfurous acid results in the formation of fuchsin sulfurous acid, also referred to as Feulgen reagent or Schiff’s reagent. This reagent is responsible for the staining of chromosomal DNA. Basic fuchsin is also used for the staining of lignin in plant tissue samples.

Suitability

Certified for use for acid-fast staining with carbol-fuchsin, as ENDO medium, and as Schiff′s reagent in the Periodic Acid Schiff (PAS) and Feulgen techniques.

Legal Information

Magenta is a trademark of Magenta Corp.

Safety & Documentation

Safety Information

Symbol 
GHS08  GHS08
Signal word 
Danger
Hazard statements 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
RTECS 
CX9850100
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles
Peer-Reviewed Papers
15

References

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