85766 Millipore

m-Endo Agar LES

for microbiology, NutriSelect Plus

Synonym: Membrane Endo Agar LES

  •  NACRES NA.85



Related Categories A - M, All Solid Media/Agars, Escherichia coli & Coliforms, Media by Application, Media by Microorganism,
Quality Level   100
grade   for microbiology
form   powder
shelf life   limited shelf life, expiry date on the label
composition   agar, 15 g/L
  casein hydrolysate, 3.7 g/L
  di-potassium hydrogen phosphate, 3.3 g/L
  lactose, 9.4 g/L
  pararosanilin (fuchsin), 0.8 g/L
  peptone from meat, 3.7 g/L
  potassium hydrogen phosphate, 1 g/L
  sodium chloride, 3.7 g/L
  sodium deoxycholate, 0.1 g/L
  sodium lauryl sulfate, 0.05 g/L
  sodium sulfite, 1.6 g/L
  tryptose, 7.5 g/L
  yeast extract, 1.2 g/L
mfr. no.   NutriSelect Plus
application(s)   bioburden testing: suitable
  microbiological culture: suitable
  water monitoring: suitable
final pH   7.2±0.2±25 °C
Featured Industry   Agriculture
Food and Beverages
suitability   selective and differential for Escherichia coli
  selective and differential for Proteus spp.
  selective and differential for Salmonella spp.
  selective and differential for coliforms



m-Endo Agar LES can be used for the enumeration of coliforms in water using the Membrane filter technique for coliform enumeration. This method is more reliable and precise than MPN multiple test. It follows the two step membrane filter procedure using Lauryl Sulfate Broth (Cat. No. 17349) as a preliminary enrichment, resulting in higher coliform counts.

Preparation Note

Suspend 51 g in 1 litre of distilled water containing 20 ml of ethanol 96 %. Boil to dissolve completely. Do not autoclave! Cool to 45-50°C and dispense 4 ml amounts into 50-60 mm Petri dishes and allow to solidify. The agar should be at least 1.5mm depth. Do not expose plates to direct sunlight.

Safety & Documentation

Safety Information

GHS08  GHS08
Signal word 
Hazard statements 
Precautionary statements 
NONH for all modes of transport
WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable


Certificate of Analysis (COA)

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Protocols & Articles


Microbiology Introduction

An initial aim of all microbiologists is the reproducible growth of their microbial cultures, no matter whether the microorganisms are of natural origin or have been genetically engineered by man. Re...
Keywords: Absorption, Aerobic, Anaerobic, Antibiotics, Antimicrobials, Buffers, Culture media, Diffusion, Environmental, Filtration, Genetic, Growth factors, Metabolism, Phase transitions, Precipitation, Reductions, Respiration, Sample preparations, Vitamins

Microbiology Theory: Media Preparation

Microorganisms need nutrients, a source of energy and certain environmental conditions in order to grow and reproduce. In the natural environment, microbes have adapted to the habitats most suitable ...
Keywords: Culture media, Digestions, Environmental, Filtration, Growth factors, Indicators, Microbiology, Phase transitions, Precipitation, Vitamins

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