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85927 Millipore

ECC ChromoSelect Selective Agar

for microbiology

Synonym: E. coli-Coliform Selective Agar ChromoSelect, ECC Selective Agar ChromoSelect



Related Categories A - M, All Food Control Media (alphabetical), All Solid Media/Agars, Chromogenic Media, Citrobacter,
Quality Level   100
grade   for microbiology
form   powder
shelf life   limited shelf life, expiry date on the label
composition   agar, 10.0 g/L
  casein enzymic hydrolysate, 3.3 g/L
  chromogenic mixture, 0.43 g/L
  disodium hydrogen phosphate, 1 g/L
  peptone, special, 6.0 g/L
  sodium chloride, 2 g/L
  sodium dihydrogen phosphate, 0.6 g/L
  sodium pyruvate, 1 g/L
  sorbitol, 1 g/L
7, 0.15 g/L
  tryptophan, 1 g/L
application(s)   bioburden testing: suitable
  microbiology: suitable (microbial identification)
  water monitoring: suitable
final pH   6.8±0.2 (25 °C)
Featured Industry   Agriculture
Food and Beverages
suitability   selective and differential for Citrobacter spp.
  selective and differential for Enterobacter spp.
  selective and differential for Escherichia coli
  selective and differential for Salmonella spp.
  selective and differential for Shigella spp.
  selective and differential for coliforms
  selective and differential for enterobacteriaceae



ECC ChromoSelect Selective Agar is a selective medium recommended for the simultaneous detection of Escherichia coli and coliforms in water and food samples. The ingredients help even the sublethally injured coliforms to grow rapidly, Tergitol inhibits Gram-positive as well as some Gram-negative bacteria other than coliforms. The chromogenic mixture contains two chromogenic substrates as Salmon-GAL and X-glucuronide. The enzyme β-D-galactosidase produced by coliforms cleaves Salmon-GAL, resulting in the salmon-to-red coloration of coliform colonies. The enzyme β-D-glucuronidase produced by E. coli, cleaves X-glucuronide. E. coli forms dark blue-to-violet colored colonies due to cleavage of both Salmon-GAL and X-glucuronide. The addition of tryptophan improves the indole reaction. To confirm E. coli, add a drop of Kovac′s reagent (Catalog No. 60983) on the dark blue-to-violet colony. Formation of cherry-red color indicates the positive reaction.

Preparation Note

Supplement: 5-10 mg/l Cefsulodin (Cat. No. 22126)


Suspend 26.5 g in 1 liter of distilled water. Heat in a boiling water bath or in a free flowing steam, with stirring to dissolve the medium completely (approximately 35 minutes). Medium may show haziness, but it does not affect the performance. If desired to inhibit Pseudomonas and Aeromonas species, add 5 mg of cefsulodin for surface and pour plate method and 10 mg of cefsulodin for membrane filter technique. Mix well.

Legal Information

TERGITOL is a trademark of The Dow Chemical Company ("Dow") or an affiliated company of Dow

Safety & Documentation

Safety Information

GHS07  GHS07
Signal word 
Hazard statements 
Precautionary statements 
Personal Protective Equipment 
NONH for all modes of transport
WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable


Certificate of Analysis (COA)

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Protocols & Articles


Microbiology Introduction

An initial aim of all microbiologists is the reproducible growth of their microbial cultures, no matter whether the microorganisms are of natural origin or have been genetically engineered by man. Re...
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Microbiology Theory: Media Preparation

Microorganisms need nutrients, a source of energy and certain environmental conditions in order to grow and reproduce. In the natural environment, microbes have adapted to the habitats most suitable ...
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Overview of Chromogenic Media

The use of traditional versus improved media formulation containing chromogenic substrate is currently an important topic in the field of microbiology. The focus behind such developments was to produ...
Jvo SiegristProduct Manager
AnalytiX Issue 3
Keywords: Culture media, Diffusion, Electron capture detector, Fermentation, Filtration, Metabolites, Microbiology

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