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861006 Sigma-Aldrich

Eosin B

certified by the Biological Stain Commission, Dye content 90 %

Synonym: 4′,5′-Dibromo-2′,7′-dinitrofluorescein disodium salt, Acid Red 91, Eosin Scarlet, Eosin bluish, Saffrosine

  • CAS Number 548-24-3

  • Linear Formula C20H6N2O9Br2Na2

  • Molecular Weight 624.06

  •  Colour Index Number 45400

  •  Beilstein/REAXYS Number 4121964

  •  EC Number 208-943-1

  •  MDL number MFCD00005041

  •  PubChem Substance ID 24888525

  •  NACRES NA.47

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Properties

Related Categories Cell Biology, Certified Stains & Dyes, E-F, Hematology and Histology, Stains & Dyes, A to Z,
Quality Level   200
grade   certified by the Biological Stain Commission
composition   Dye content, 90%
λmax   395 nm
  514 nm
SMILES string   [Na+].[Na+].[O-]C(=O)c1ccccc1C2=C3C=C(C(=O)C(Br)=C3Oc4c(Br)c([O-])c(cc24)[N+]([O-])=O)[N+]([O-])=O
InChI   1S/C20H8Br2N2O9.2Na/c21-14-16(25)11(23(29)30)5-9-13(7-3-1-2-4-8(7)20(27)28)10-6-12(24(31)32)17(26)15(22)19(10)33-18(9)14;;/h1-6,25H,(H,27,28);;/q;2*+1/p-2
InChI key   GYYTYUGGVBYJHE-UHFFFAOYSA-L

Description

Application

• Eosin B has been used for the histological analysis of anal sphincter sections obtained from rats.
• It has been used along with Mayer′s hematoxylin for the staining of triangularis sterni muscle sections.
• It has been used along with hematoxylin for the staining of different tissue sections obtained from mouse.

Packaging

10, 25, 100 g in glass bottle

Biochem/physiol Actions

Eosin B is a xanthene dye and is also referred to as eosin bluish or erythrosin B. It is used in the Harris stain for Negri bodies. Eosin B is also used in Mayer’s Hemalum as a counterstain for collagen. Eosin B-Azure A are used together for the staining of cell granules, nuclei and microorganisms. It can also be used for protein estimation. The protein - eosin B complex absorbs at wavelength range of 536–544nm and the change in blue shift helps in determining the protein concentration.

Suitability

Counterstain for collagen, following Mayer′s Hemalum. Used with Azure A as a tissue stain for cell granules, nuclei, and microorganisms. For differential staining of cells of the anterior pituitary.

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles
Peer-Reviewed Papers
15

References

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