EMAIL THIS PAGE TO A FRIEND
89610 Sigma-Aldrich

o-Toluidine

purum p.a., ≥99.5% (GC)

Synonym: 2-Aminotoluene, 2-Methylaniline

  • CAS Number 95-53-4

  • Linear Formula CH3C6H4NH2

  • Molecular Weight 107.15

  •  Beilstein/REAXYS Number 741981

  •  EC Number 202-429-0

  •  MDL number MFCD00007730

  •  PubChem Substance ID 329769755

  •  NACRES NA.22

Purchase

Properties

Related Categories Analytical Reagents, Analytical/Chromatography, Derivatization Reagents, Derivatization Reagents TLC, TLC Reagents, S-Z,
Quality Level   100
vapor density   3.7 (vs air)
vapor pressure   0.26 mmHg ( 25 °C)
grade   purum p.a.
assay   ≥99.5% (GC)
form   liquid
autoignition temp.   899 °F
impurities   ≤0.1% water
  ≤0.5% m- and p-toluidine (GC)
ign. residue   ≤0.01% (as SO4)
refractive index   n20/D 1.572 (lit.)
  n20/D 1.572
bp   199-200 °C (lit.)
  89-90 °C/11 mmHg (lit.)
mp   −28 °C (lit.)
solubility   H2O: slightly soluble
density   1.008 g/mL at 25 °C (lit.)
cation traces   Ca: ≤5 mg/kg
  Cd: ≤1 mg/kg
  Co: ≤1 mg/kg
  Cr: ≤1 mg/kg
  Cu: ≤1 mg/kg
  Fe: ≤1 mg/kg
  K: ≤20 mg/kg
  Mg: ≤1 mg/kg
  Mn: ≤1 mg/kg
  Na: ≤20 mg/kg
  Ni: ≤1 mg/kg
  Pb: ≤1 mg/kg
  Zn: ≤1 mg/kg
SMILES string   Cc1ccccc1N
InChI   1S/C7H9N/c1-6-4-2-3-5-7(6)8/h2-5H,8H2,1H3
InChI key   RNVCVTLRINQCPJ-UHFFFAOYSA-N

Description

General description

o-Toluidine belongs to the class of monocyclic aromatic amines, which are of research and industrial importance. It can be used as a derivatization agent for the resolution of analytes by the formation of chromophores, which can be visualized by UV radiation or fluorescence.

Application

o-Toluidine may be used as a TLC Visualization (Staining) Reagent for the detection of reducing sugars in protein hydrolysates by using high-performance thin-layer chromatography (HPTLC) coupled with UV diffuse reflectance scanning densitometry.

Caution

may discolor on storage

Safety & Documentation

Safety Information

Signal word 
Danger
Hazard statements 
RIDADR 
UN 1708 6.1 / PGII
WGK Germany 
WGK 3
RTECS 
XU2975000
Flash Point(F) 
185.0 °F - closed cup
Flash Point(C) 
85 °C - closed cup
Protocols & Articles

Articles

GC Analysis of Anilines on Equity®-5

From our library of Articles, Sigma-Aldrich presents GC Analysis of Anilines on Equity®-5
Keywords: Chromatography, Flame ionization detector, Gas chromatography, Purification

GC Analysis of Semivolatiles on SLB®-5ms after SPME using 60 μm Carbowax® Fiber

From our library of Articles, Sigma-Aldrich presents GC Analysis of Semivolatiles on SLB®-5ms after SPME using 60 μm Carbowax® Fiber
Keywords: Chromatography, Gas chromatography, Purification, Solid phase microextractions

GC Analysis of a 16-Component Anilines Mix on SLB®-IL59

From our library of Articles, Sigma-Aldrich presents GC Analysis of a 16-Component Anilines Mix on SLB®-IL59
Keywords: Chromatography, Gas chromatography

GC Analysis of a 32-Component Anilines Mix on SLB®-IL59

From our library of Articles, Sigma-Aldrich presents GC Analysis of a 32-Component Anilines Mix on SLB®-IL59
Keywords: Chromatography, Gas chromatography, Mass selective detector

HPLC Analysis of Toluidine Isomers on Ascentis® Si (Hexane: 2-Propanol)

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Toluidine Isomers on Ascentis® Si (Hexane: 2-Propanol)
Keywords: Chromatography, High performance liquid chromatography

HPLC Analysis of Toluidine Isomers on Ascentis® Si (Silica), (80:20 Hexane:Ethyl Acetate)

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Toluidine Isomers on Ascentis® Si (Silica), (80:20 Hexane:Ethyl Acetate)
Keywords: Chromatography, High performance liquid chromatography

US EPA Method 8270 (Appendix IX): GC Analysis of Semivolatiles on Equity®-5 (30 m x 0.25 mm I.D., 0.50 μm)

From our library of Articles, Sigma-Aldrich presents US EPA Method 8270 (Appendix IX): GC Analysis of Semivolatiles on Equity®-5 (30 m x 0.25 mm I.D., 0.50 μm)
Keywords: Chromatography, Gas chromatography, Mass selective detector, Purification

Peer-Reviewed Papers
15

References

Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?