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900667 Sigma-Aldrich

Acetonitrile

for UHPLC, for mass spectrometry

Synonym: ACN, Cyanomethane, Ethyl nitrile, Methyl cyanide

  • CAS Number 75-05-8

  • Linear Formula CH3CN

  • Molecular Weight 41.05

  •  Beilstein/REAXYS Number 741857

  •  EC Number 200-835-2

  •  MDL number MFCD00001878

  •  NACRES NA.21

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Properties

Related Categories Analytical Reagents, Analytical/Chromatography, Chromatography Reagents & Solvents, GC Solvents, Solvents for GC applications,
Quality Level   100
grade   for mass spectrometry
vapor density   1.41 (vs air)
vapor pressure   72.8 mmHg ( 20 °C)
assay   ≥99.92%
autoignition temp.   973 °F
expl. lim.   16 %
refractive index   n20/D 1.344 (lit.)
bp   81-82 °C (lit.)
mp   −45 °C (lit.)
density   0.786 g/mL at 25 °C (lit.)
Featured Industry   Food and Beverages
SMILES string   CC#N
InChI   1S/C2H3N/c1-2-3/h1H3
InChI key   WEVYAHXRMPXWCK-UHFFFAOYSA-N

Description

General description

Acetonitrile, an aliphatic nitrile, is a highly volatile, aprotic polar organic solvent that finds wide use in analytical determinations. It is UV-transparent − a property that facilitates its utilization in spectrophotometric and fluorimetric analyses. Its low viscosity, high elution strength and miscibility in water makes it viable in many chromatography systems.
Its other applications include its use as a mobile phase additive and as an extraction solvent in liquid-liquid extraction (LLC), solid-phase extraction (SPE) and microextraction for chromatographic applications, etc.

Application

Acetonitrile may be used:
• As an extraction solvent for the quantification of pesticide analytes in foodstuffs and tea, industrial dyes in juice and wine by ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS).
• As an organic modifier in the determination of bisphenol A-diglycidyl ether, bisphenol F-diglycidyl ether and their derivatives in canned food and beverages by fast LC–MS/MS.

Acetonitrile, an organic solvent, is used as a mobile phase/extraction solvent in the ultra-high performance liquid chromatorgraphy − tandem mass spectrometry (UHPLC-MS) analysis of pesticides in tobacco samples. It can be potentially used with a reverse-phase column to determine the concentration of diclofenac (DCF).

Packaging

1 L in glass bottle

100, 250 mL in glass bottle

Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
RIDADR 
UN 1648 3 / PGII
WGK Germany 
WGK 2
RTECS 
AL7700000
Flash Point(F) 
35.6 °F - closed cup
Flash Point(C) 
2.0 °C - closed cup

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

Articles

Reversed-Phase HPLC Analysis of Insulin Variants and Analogs by UV and Mass Spectral Detection

Insulin is an approximately 6000 Da peptide hormone used to regulate the glucose concentration in blood. There are several different forms of insulin that are both natural and recombinant; the recomb...
Cory Muraco, Stacy Shollenberger, and Hillel Brandes
MilliporeSigma, Bellefonte, PA USA
Keywords: Chromatography, High performance liquid chromatography, Hormones, Mass spectrometry, Pharmaceutical, Phase transitions, Reversed-phase chromatography, Separation

Peer-Reviewed Papers
15

References

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