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  • 90404 - Thioglycolate Broth with Resazurine

90404 Millipore

Thioglycolate Broth with Resazurine

for microbiology, NutriSelect Plus

Synonym: Fluid Thioglycolate Medium

  •  NACRES NA.85



Related Categories All Food Control Media (alphabetical), All Liquid Media/Broths, Bacillus, Bacteria (General Media), Bacteroides,
Quality Level   100
grade   for microbiology
form   powder
shelf life   limited shelf life, expiry date on the label
composition   agar, 0.75 g/L
  casein enzymic hydrolysate, 15.00 g/L
  L-cystine, 0.50 g/L
  dextrose, 5.50 g/L
  resazurine, 0.001 g/L
  sodium chloride, 2.50 g/L
  sodium thioglycolate, 0.50 g/L
  yeast extract, 5.00 g/L
mfr. no.   NutriSelect Plus
application(s)   microbiological culture: suitable
  sterility testing: suitable
final pH   7.1±0.2
Featured Industry   Agriculture
Clinical Testing
Food and Beverages
suitability   Micrococcus spp.
  nonselective for Bacillus spp.
  nonselective for Bacteroides spp.
  nonselective for Candida spp.
  nonselective for Clostridium spp.
  nonselective for Neisseria spp.
  nonselective for Streptococcus spp.
  nonselective for bacteria (General Media)



For sterility testing of biologicals and for cultivation of aerobic and anaerobic organisms. Any increase in the oxygen content is indicated by a color change of redox indicator resazurin to red.

Preparation Note

Suspend 29.75 g in 1000 ml distilled water. Boil to dissolve the medium completely. Sterilize by autoclaving at 121°C for 15 minutes. Cool to 25°C and store in a cool dark place preferably below 25°C.

Other Notes

Medium for aerobes and anaerobes

Safety & Documentation

Safety Information

Personal Protective Equipment 
NONH for all modes of transport
WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable


Certificate of Analysis (COA)

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Protocols & Articles


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Microbiology Theory: Media Preparation

Microorganisms need nutrients, a source of energy and certain environmental conditions in order to grow and reproduce. In the natural environment, microbes have adapted to the habitats most suitable ...
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Streptococci are non-motile, microaerophilic, Grampositive spherical bacteria (cocci). They often occur as chains or pairs and are facultative or strict anaerobes. Streptococci give a negative catala...
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Keywords: Cell division, Diagnostic, Phosphorylations

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