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92499 Millipore

Triple Sugar Iron Agar (acc. to ISO)

suitable for microbiology, NutriSelect® Plus

Synonym: TSI Agar

  •  NACRES NA.85

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Properties

Related Categories All Dairy Media (alphabetical), All Food Control Media (alphabetical), All Solid Media/Agars, Citrobacter, Clinical Media,
Quality Level   100
form   powder
shelf life   limited shelf life, expiry date on the label
composition   agar, 12 g/L
  dextrose, 1 g/L
  ferric ammonium citrate, 0.3 g/L
  lactose, 10 g/L
  meat extract, 3 g/L
  peptone, 20 g/L
  phenol red, 0.025 g/L
  sodium chloride, 5 g/L
  sodium thiosulfate, 0.3 g/L
  sucrose, 10 g/L
  yeast extract, 3 g/L
mfr. no.   NutriSelect® Plus
application(s)   microbiological culture: suitable
  microbiology: suitable (microbial identification)
final pH   7.4±0.2 (25 °C)
Featured Industry   Clinical Testing
Environmental
Food and Beverages
Pharmaceutical
suitability   nonselective and differential for Citrobacter spp.
  nonselective and differential for Escherichia coli
  nonselective and differential for Klebsiella spp.
  nonselective and differential for Proteus spp.
  nonselective and differential for Salmonella spp.
  nonselective and differential for Shigella spp.
  nonselective and differential for coliforms
  nonselective and differential for enterobacteriaceae

Description

Application

Differential medium for identification of enterobacteria, according to ISO 6579, 6785 and 10272 Standards.

Preparation Note

Dissolve 64.6g of powder in 1 L of distilled water and bring to boiling. Dispense into tubes and sterilize at 121°C for 15 minutes. Leave to solidify with short slant and good butts.

Legal Information

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Microbiology Introduction

An initial aim of all microbiologists is the reproducible growth of their microbial cultures, no matter whether the microorganisms are of natural origin or have been genetically engineered by man. Re...
Keywords: Absorption, Aerobic, Anaerobic, Antibiotics, Antimicrobials, Buffers, Culture media, Diffusion, Environmental, Filtration, Genetic, Growth factors, Metabolism, Phase transitions, Precipitation, Reductions, Respiration, Sample preparations, Vitamins

Microbiology Theory: Media Preparation

Microorganisms need nutrients, a source of energy and certain environmental conditions in order to grow and reproduce. In the natural environment, microbes have adapted to the habitats most suitable ...
Keywords: Culture media, Digestions, Environmental, Filtration, Growth factors, Indicators, Microbiology, Phase transitions, Precipitation, Vitamins

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