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Trizma® base

certified reference material for titrimetry, certified by BAM, ≥99.5%

Synonym: 2-Amino-2-(hydroxymethyl)-1,3-propanediol, THAM, Tris base, Tris(hydroxymethyl)aminomethane, Trometamol

  • CAS Number 77-86-1

  • Linear Formula NH2C(CH2OH)3

  • Molecular Weight 121.14

  •  Beilstein/REAXYS Number 741883

  •  EC Number 201-064-4

  •  MDL number MFCD00004679

  •  PubChem Substance ID 329770235

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Properties

Related Categories Additional Standards, Analytical Standards, Analytical/Chromatography, Application CRMs, Certified Reference Materials (CRMs),
grade   certified reference material
description   aminopeptidase substrate
InChI Key   LENZDBCJOHFCAS-UHFFFAOYSA-N
assay   ≥99.5%
form   neat
quality   certified by BAM
application(s)   titration: suitable
pH   10.5-12
useful pH range   7 - 9
pKa (25 °C)   8.1
bp   219-220 °C/10 mmHg(lit.)
mp   167-172 °C (lit.)
solubility   water: soluble (678 g/l at 20 °C)
format   neat

Description

Application

Trizma® base may be used in the extraction and isolation of high quality DNA from plant tissues using cetyltrimethylammonium bromide as the extracting agent, analyzed using agarose gel electrophoresis technique.

Analysis Note

Exact concentration, expiry date, traceability and detailed information on certification can be found on the certificate and certification report, included in each package. Content and expiry date on label.

Other Notes

The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

Legal Information

Trizma is a registered trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
2
RTECS 
TY2900000

Documents

Certificate of Analysis

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Protocols & Articles
Peer-Reviewed Papers
15

References

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